Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:227089
Pirson M, et al., Expression of peroxiredoxins and thioredoxins in the mouse spinal cord during embryonic development. J Comp Neurol. 2015 Dec 1;523(17):2599-617
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6144137
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Gene symbol:
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Prdx3
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Gene name:
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peroxiredoxin 3
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Antibody:
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anti-Prdx3
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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|
Results
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Specimen
1C:
embryonic day 16.5
(more )
Specimen
1C:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
2B/D:
embryonic day 16.5
(more )
Note: Double labeled: green - Prdx3; red - Atp1b1 (assay MGI:6144143).
Specimen
2B/D:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Prdx3; red - Atp1b1 (assay MGI:6144143).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spinal cord |
Present |
Punctate |
2D
,
2B
|
Authors report punctate expression and co-localization with mitochondrial marker, Atpb.
|
Specimen
5A:
embryonic day 10.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5B:
embryonic day 11.5
(more )
Specimen
5B:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Strong |
Regionally restricted |
5B
|
Authors state increased ventral expression persisted until E18.5.
|
Specimen
5C:
embryonic day 12.5
(more )
Specimen
5C:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Strong |
Regionally restricted |
5C
|
Authors state increased ventral expression persisted until E18.5.
|
Specimen
5D:
embryonic day 13.5
(more )
Specimen
5D:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5E:
embryonic day 14.5
(more )
Specimen
5E:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: spinal cord |
Strong |
Regionally restricted |
5E
|
Authors state increased ventral expression persisted until E18.5.
|
Specimen
5F:
embryonic day 16.5
(more )
Specimen
5F:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spinal cord |
Present |
|
5F
|
Authors report expression is relatively less striking at E16.5-E18.5.
|
Specimen
5G:
embryonic day 18.5
(more )
Specimen
5G:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: spinal cord |
Present |
|
5G
|
Authors report expression is relatively less striking at E16.5-E18.5.
|
Specimen
5H/K:
embryonic day 12.5
(more )
Note: Triple labeled: green - Prdx3; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
Specimen
5H/K:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Prdx3; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Strong |
Regionally restricted |
5H
,
5K
|
Authors report strong ventral expression co-localized in nucleus with motor neuron markers Isl1/2 and Foxp1.
|
Specimen
5L/O:
embryonic day 12.5
(more )
Note: Triple labeled: green - Prdx3; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
Specimen
5L/O:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Prdx3; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Punctate |
5O
,
5L
|
Punctuated pattern of expression typical of mitochondrial localization.
|
Specimen
5P/S:
embryonic day 16.5
(more )
Note: Triple labeled: green - Prdx3; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
Specimen
5P/S:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Prdx3; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spinal cord |
Strong |
Regionally restricted |
5S
,
5P
|
Authors report strong ventral expression co-localized in nucleus with motor neuron markers Isl1/2 and Foxp1.
|
Specimen
5T/W:
embryonic day 16.5
(more )
Note: Triple labeled: green - Prdx3; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
Specimen
5T/W:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Prdx3; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spinal cord |
Present |
Punctate |
5T
,
5W
|
Punctuated pattern of expression typical of mitochondrial localization.
|
|