Gene Expression Data
Assay Details
Assay
|
Reference:
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J:227089
Pirson M, et al., Expression of peroxiredoxins and thioredoxins in the mouse spinal cord during embryonic development. J Comp Neurol. 2015 Dec 1;523(17):2599-617
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6144135
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Gene symbol:
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Prdx1
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Gene name:
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peroxiredoxin 1
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Antibody:
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anti-Prdx1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3A:
embryonic day 10.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
C57BL/6N
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Age:
|
embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Present |
Regionally restricted |
3A
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Distinct nuclear localization of expression in entire spinal cord. Slightly stronger expression in ventral lateral areas.
|
Specimen
3B:
embryonic day 11.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
C57BL/6N
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Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Present |
Regionally restricted |
3B
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Intense nuclear expression in ventral areas; more diffuse expression in area adjacent to ventricular zone.
|
TS19: dorsal root ganglion |
Strong |
Regionally restricted |
3B
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Strong expression in nucleus of dorsal root ganglia cells.
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Specimen
3C:
embryonic day 12.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
C57BL/6N
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Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3D:
embryonic day 13.5
(more )
Specimen
3D:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Widespread |
3D
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Predominantly nuclear expression covering entire spinal cord.
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Specimen
3E:
embryonic day 14.5
(more )
Specimen
3E:
(close )
Genetic Background:
|
C57BL/6N
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Age:
|
embryonic day 14.5
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3F:
embryonic day 16.5
(more )
Specimen
3F:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spinal cord |
Strong |
Regionally restricted |
3F
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Intense nuclear expression in a ventrally-located cell population. Weak cytosolic expression over rest of spinal cord - stronger in most rostral part of spinal cord.
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Specimen
3G:
embryonic day 18.5
(more )
Specimen
3G:
(close )
Genetic Background:
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C57BL/6N
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: spinal cord |
Strong |
Regionally restricted |
3G
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Intense nuclear expression in ventrally-located cell population. Weak cytosolic expression over rest of spinal cord.
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Specimen
3H/K:
embryonic day 12.5
(more )
Note: Triple labeled: green - Prdx1; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
Specimen
3H/K:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Prdx1; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Strong |
Regionally restricted |
3H
,
3K
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Authors report strong ventral expression co-localized in the nucleus with motor neuron markers Isl1/2 and Foxp1.
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Specimen
3L/O:
embryonic day 12.5
(more )
Note: Triple labeled: green - Prdx1; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
Specimen
3L/O:
(close )
Genetic Background:
|
C57BL/6N
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Prdx1; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Strong |
Regionally restricted |
3O
,
3L
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Authors report strong ventral expression co-localized in the nucleus with motor neuron markers Isl1/2 and Foxp1.
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Specimen
3P/S:
embryonic day 16.5
(more )
Note: Triple labeled: green - Prdx1; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
Specimen
3P/S:
(close )
Genetic Background:
|
C57BL/6N
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Age:
|
embryonic day 16.5
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Prdx1; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spinal cord |
Strong |
Regionally restricted |
3S
,
3P
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Authors report strong ventral expression co-localized in the nucleus with motor neuron markers Isl1/2 and Foxp1.
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Specimen
3T/W:
embryonic day 16.5
(more )
Note: Triple labeled: green - Prdx1; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
Specimen
3T/W:
(close )
Genetic Background:
|
C57BL/6N
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Age:
|
embryonic day 16.5
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Prdx1; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spinal cord |
Strong |
Regionally restricted |
3W
,
3T
|
Authors report strong ventral expression co-localized in the nucleus with motor neuron markers Isl1/2 and Foxp1.
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