Antibody:	anti-Oct4 (N-19; sc-8628)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS12: embryo	Present		1A

Structure	Level	Pattern	Image	Note
TS12: surface ectoderm	Present		1B
TS12: neural ectoderm	Present	Regionally restricted	1B	Expression is in the anterior neurectoderm.

Structure	Level	Pattern	Image	Note
TS12: surface ectoderm	Present		1C
TS12: neural ectoderm	Present	Regionally restricted	1C	Expression is in the anterior neurectoderm.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present		1F

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present		1G

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present		1H

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present		1I

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present		2A
TS10: extraembryonic mesoderm	Present		2A
TS10: primitive streak	Present		2A
TS10: embryo	Present	Regionally restricted	2A	Expression is in the epiblast.
TS10: primitive endoderm	Present	Regionally restricted	2A	Expression extended circumferentially around the egg cylinder from the anterior level of the primitive streak to the distal tip of the egg cylinder.
TS10: extraembryonic component	Present	Regionally restricted	2A	Aside from the exraembryonic mesoderm all other extraembryonic tissues had no expression.
TS10: extraembryonic ectoderm	Absent		2A
TS10: extraembryonic endoderm	Absent		2A

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present		2B
TS10: extraembryonic mesoderm	Present		2B
TS10: primitive streak	Present		2B
TS10: embryo	Present	Regionally restricted	2B	Expression is in the epiblast.
TS10: primitive endoderm	Present	Regionally restricted	2B	Expression extended circumferentially around the egg cylinder from the anterior level of the primitive streak to the distal tip of the egg cylinder.
TS10: extraembryonic component	Present	Regionally restricted	2B	Aside from the exraembryonic mesoderm all other extraembryonic tissues had no expression.
TS10: extraembryonic ectoderm	Absent		2B
TS10: extraembryonic endoderm	Absent		2B

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present		2C
TS10: extraembryonic mesoderm	Present		2C
TS10: primitive streak	Present		2C
TS10: embryo	Present	Regionally restricted	2C	Expression is in the epiblast.
TS10: primitive endoderm	Present	Regionally restricted	2C	Expression extended circumferentially around the egg cylinder from the anterior level of the primitive streak to the distal tip of the egg cylinder.
TS10: extraembryonic component	Present	Regionally restricted	2C	Aside from the exraembryonic mesoderm all other extraembryonic tissues had no expression.
TS10: extraembryonic ectoderm	Absent		2C
TS10: extraembryonic endoderm	Absent		2C

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present		2D
TS10: extraembryonic mesoderm	Present		2D
TS10: node	Present		2D
TS10: primitive streak	Present		2D
TS10: embryo	Present	Regionally restricted	2D	Expression is in the epiblast.
TS10: primitive endoderm	Present	Regionally restricted	2D	Positive cells are associated with the full circumference of paraxial and lateral plate mesoderm.
TS10: extraembryonic component	Present	Regionally restricted	2D	Aside from the exraembryonic mesoderm all other extraembryonic tissues had no expression.
TS10: extraembryonic ectoderm	Absent		2D
TS10: extraembryonic endoderm	Absent		2D

Structure	Level	Pattern	Image	Note
TS11: notochordal process	Very strong		2E
TS11: embryo mesenchyme	Very strong	Regionally restricted	2E	Expression is in nascent prospective paraxial mesoderm.
TS11: lateral plate mesoderm	Very strong	Regionally restricted	2E	Expression is in the posterior lateral plate mesoderm. The anterior half varied from slightly positive to negative in expression.
TS11: primitive endoderm	Present	Regionally restricted	2E	Expression is in cells overlying prospective paraxial and lateral plate mesoderm.
TS11: node	Present		2E

Structure	Level	Pattern	Image	Note
TS11: embryo mesenchyme	Very strong	Regionally restricted	2F	Expression is in nascent prospective paraxial mesoderm.
TS11: lateral plate mesoderm	Very strong	Regionally restricted	2F	Expression is in the posterior lateral plate mesoderm.

Structure	Level	Pattern	Image	Note
TS11: amnion ectoderm	Present		2G
TS11: exocoelomic cavity	Present	Regionally restricted	2G	Expression is in mesoderm cells lining the nascent exocoelom.
TS11: mesoderm	Present	Regionally restricted	2G	Expression is in mesoderm cells lining the nascent exocoelom.
TS11: extraembryonic endoderm	Absent		2G

Structure	Level	Pattern	Image	Note
TS11: node	Strong		2H
TS11: primitive streak	Strong		2H
TS11: notochordal process	Present	Regionally restricted	2H	Expression is reduced to absent anteriorly.

Structure	Level	Pattern	Image	Note
TS11: primitive endoderm	Present	Regionally restricted	2I	Expression is in cells overlying prospective paraxial and lateral plate mesoderm and associated with the posterior primitive streak.
TS11: neural plate	Present		2I
TS11: notochordal process	Present		2I
TS11: embryo mesenchyme	Strong	Regionally restricted	2I	Expression is in the paraxial mesoderm.

Structure	Level	Pattern	Image	Note
TS11: primitive endoderm	Present	Regionally restricted	2J	Expression is in cells overlying prospective paraxial and lateral plate mesoderm.
TS11: notochordal process	Present		2J
TS11: lateral plate mesoderm	Present	Uniform	2J

Structure	Level	Pattern	Image	Note
TS11: exocoelomic cavity	Present	Regionally restricted	3A	Expression is in mesoderm cells lining the exocoelom.
TS11: extraembryonic mesoderm	Present	Regionally restricted	3A	Expression is in mesoderm cells lining the exocoelom.
TS11: allantois	Present	Regionally restricted	3A	Expression is in the inner and outer allantoic cell population.
TS11: amnion ectoderm	Present		3A
TS11: blood island	Absent		3A
TS11: extraembryonic visceral endoderm	Absent		3A	Expression is absent in the allantois-associated extraembryonic visceral endoderm.

Structure	Level	Pattern	Image	Note
TS11: exocoelomic cavity	Present	Regionally restricted	3B	Expression is in mesoderm cells lining the exocoelom.
TS11: extraembryonic mesoderm	Present	Regionally restricted	3B	Expression is in mesoderm cells lining the exocoelom.
TS11: allantois	Present	Regionally restricted	3B	Expression is in the inner and outer allantoic cell population.
TS11: amnion ectoderm	Present		3B
TS11: blood island	Absent		3B
TS11: extraembryonic visceral endoderm	Absent		3B	Expression is absent in the allantois-associated extraembryonic visceral endoderm.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Regionally restricted	3C	Expression is in the inner and occasionally outer associated cells of the allantois.
TS12: extraembryonic visceral endoderm	Present	Regionally restricted	3C	Expression is in the allantois-associated extraembryonic visceral endoderm.
TS12: primitive endoderm	Present	Regionally restricted	3C	Expression is in the endoderm overlying the posterior primitive streak.
TS12: amnion ectoderm	Present		3C

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Regionally restricted	3D	The expression domain is symmetric about the allantois' putative A-P axis, but asymmetric with respect to its dorsalventral (D-V) axis.
TS12: amnion ectoderm	Present		3D
TS12: extraembryonic visceral endoderm	Present	Regionally restricted	3D	Expression is in the allantis-associated extraembryonic visceral endoderm.

Structure	Level	Pattern	Image	Note
TS13: allantois	Present		3E
TS13: extraembryonic endoderm	Present	Regionally restricted	3E	Expression is in the allantis-associated extraembryonic visceral endoderm.
TS13: alimentary system endoderm	Present	Regionally restricted	3E	Expression is in the embryonic visceral endoderm overlying the hindgut.
TS13: primitive hindgut	Present	Regionally restricted	3E	Expression is in nascent hindgut endoderm located beneath the allantois.
TS13: amnion ectoderm	Present		3E

Structure	Level	Pattern	Image	Note
TS13: extraembryonic endoderm	Present	Regionally restricted	3F	Expression is in the allantis-associated extraembryonic visceral endoderm.
TS13: allantois	Present		3F
TS13: amnion ectoderm	Present		3F
TS13: umbilical blood vessel	Present		3F

Structure	Level	Pattern	Image	Note
TS12: allantois	Present		3G
TS12: extraembryonic visceral endoderm	Present	Regionally restricted	3G	Expression is in the allantis-associated extraembryonic visceral endoderm.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present		3H

Structure	Level	Pattern	Image	Note
TS12: allantois	Present		3I

Structure	Level	Pattern	Image	Note
TS12: allantois	Present		3J

Structure	Level	Pattern	Image	Note
TS12: node	Present	Regionally restricted	5A	Expression is absent in the ventral anterior aspect.
TS12: embryo	Present	Regionally restricted	5A	Expression is in the posterior half.
TS12: posterior visceral endoderm	Present	Regionally restricted	5A	Expression is in the posterior half.
TS12: primitive streak	Present	Regionally restricted	5A	Expression is in the posterior primitive streak.

Structure	Level	Pattern	Image	Note
TS12: node	Present	Regionally restricted	5B	Expression is absent in the ventral anterior aspect.
TS12: embryo	Present	Regionally restricted	5B	Expression is in the posterior half.
TS12: posterior visceral endoderm	Present	Regionally restricted	5B	Expression is in the posterior half.
TS12: primitive streak	Present	Regionally restricted	5B	Expression is in the posterior primitive streak.

Structure	Level	Pattern	Image	Note
TS12: node	Present	Regionally restricted	5C	Expression is absent in the ventral anterior aspect.

Structure	Level	Pattern	Image	Note
TS13: node	Present	Regionally restricted	5D	Expression is dorsal and absent in the ventral node.

Structure	Level	Pattern	Image	Note
TS12: node	Present	Regionally restricted	5E	Expression is dorsal and absent in the ventral node.

Structure	Level	Pattern	Image	Note
TS12: node	Present	Regionally restricted	5F	Expression is dorsal and absent in the ventral node.
TS12: primitive endoderm	Present	Regionally restricted	5F	Expression is in the endoderm adjacent to the node.

Structure	Level	Pattern	Image	Note
TS12: primitive endoderm	Strong	Regionally restricted	5G	Expression is in the endoderm adjacent to the node.
TS12: node	Present		5G

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present		5H
TS12: primitive endoderm	Present	Regionally restricted	5H	Expression is in the endoderm adjacent to the primitive streak.

Structure	Level	Pattern	Image	Note
TS13: allantois	Strong		6A
TS13: amnion	Strong		6A
TS13: neural ectoderm	Strong		6A
TS13: embryo	Strong	Regionally restricted	6A	Expression is in posterior structures.
TS13: extraembryonic endoderm	Present	Regionally restricted	6A	Expression is in cells in the allantis-associated extraembryonic visceral endoderm overlying the primary umbilical vessel.
TS13: somite	Present	Regionally restricted	6A	Expression in each somite decreased in a rostral-caudal gradient.
TS13: heart	Absent		6A

Structure	Level	Pattern	Image	Note
TS13: somite	Present		6B	Expression is stronger in the most recently formed posterior somite.

Structure	Level	Pattern	Image	Note
TS14: neural ectoderm	Present		6C	Expression is stronger in the posterior neurectoderm.

Structure	Level	Pattern	Image	Note
TS14: neural ectoderm	Present		6D	Expression is strong in the posterior neurectoderm and weak in the anterior neurectoderm.
TS14: primitive hindgut	Present	Regionally restricted	6D	Expressing cells are in the ventral region.

Structure	Level	Pattern	Image	Note
TS14: primordial germ cell of hindgut	Present		6E	Expressing cells are in the ventral region of the hindgut.
TS14: embryo	Present	Regionally restricted	6E	There is a near global extinction of expression.

Structure	Level	Pattern	Image	Note
TS14: primordial germ cell of hindgut	Present		6F
TS14: surface ectoderm	Present	Single cells	6F
TS14: allantois	Present	Clusters	6F

Structure	Level	Pattern	Image	Note
TS14: primordial germ cell of hindgut	Present	Clusters	6G

Structure	Level	Pattern	Image	Note
TS14: allantois	Present	Clusters	6H