Antibody:	Anti-Zac1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Fixative was 4% paraformaldehyde/0.02% glutaraldehyde with a post-fix in 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS18: cerebellum primordium	Present	Regionally restricted	1A	Expression is in the cerebellar ventricular zone overlying the fourth ventricle.
TS18: rhombic lip	Absent		1A

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present	Regionally restricted	1C	Expression is in the ventricular zone.
TS20: hindbrain	Present	Regionally restricted	1C	Expression is in a stream of cells migrating dorsally from the ventricular zone towards the cortical transitory zone.

Structure	Level	Pattern	Image	Note
TS21: cerebellum ventricular layer	Present		1E
TS21: cerebellum	Present	Regionally restricted	1E	Expression is in the cortical transitory zone in a discrete cluster in the center.

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present	Regionally restricted	1G	Expression is in a subdivision of the medial cerebellar nuclei.
TS27: cerebellum ventricular layer	Present		1G

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Present	Regionally restricted	1I	Expression is in a subdivision of the medial cerebellar nuclei.
TS26: cerebellum ventricular layer	Present	Regionally restricted	1I	Expression is in ventricular zone progenitors.
TS26: cerebellar cortex	Present	Regionally restricted	1I	Expression is in cells migrating from the outer layer of the external granule layer into the immature molecular layer.
TS26: cerebellum external granule cell layer	Present	Regionally restricted	1I	Expression is in cells migrating from the outer layer of the external granule layer into the immature molecular layer.

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present	Regionally restricted	1J	Expression is in a subdivision of the medial cerebellar nuclei.
TS27: cerebellum external granule cell layer	Present	Regionally restricted	1J	Expression is in the granule layers of lobules IX and X.
TS27: cerebellum vermis lobule IX	Present	Regionally restricted	1J	Expression is in the granule layers of lobules IX and X.
TS27: cerebellum vermis lobule X	Present	Regionally restricted	1J	Expression is in the granule layers of lobules IX and X.
TS27: cerebellum ventricular layer	Absent		1J

Structure	Level	Pattern	Image	Note
TS24: cerebellum external granule cell layer	Present		2A

Structure	Level	Pattern	Image	Note
TS26: cerebellar cortex	Present	Regionally restricted	2B	Expression is in cells migrating from the outer layer of the external granule layer into the immature molecular layer.
TS26: cerebellum external granule cell layer	Present	Regionally restricted	2B	Expression is in cells migrating from the outer layer of the external granule layer into the immature molecular layer.

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Present	Regionally restricted	2C	Expression is in the granule layers of lobules IX and X.
TS27: cerebellum vermis lobule IX	Present	Regionally restricted	2C	Expression is in the granule layers of lobules IX and X.
TS27: cerebellum vermis lobule X	Present	Regionally restricted	2C	Expression is in the granule layers of lobules IX and X.

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Present	Regionally restricted	2D	Expression is in the granule layers of lobules IX and X.
TS27: cerebellum vermis lobule IX	Present	Regionally restricted	2D	Expression is in the granule layers of lobules IX and X.
TS27: cerebellum vermis lobule X	Present	Regionally restricted	2D	Expression is in the granule layers of lobules IX and X.

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present		2E	Expression is in the granule layers of lobules IX and X.
TS28: cerebellum vermis lobule IX	Present		2E	Expression is in the granule layers of lobules IX and X.
TS28: cerebellum vermis lobule X	Present		2E	Expression is in the granule layers of lobules IX and X.

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Trace		2F

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Absent		2G
TS28: cerebellum external granule cell layer	Absent		2G

Structure	Level	Pattern	Image	Note
TS26: cerebellum external granule cell layer	Present		2L

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present	Single cells	3J	Expression is in a subset of Golgi cells.
TS28: cerebellum vermis	Present	Single cells	3J	Expression is in a subset of Golgi cells.

Structure	Level	Pattern	Image	Note
TS26: cerebellum external granule cell layer	Strong	Regionally restricted	4C , 4B , 4C' , 4A	Expression is in the posterior external granule layer and in inwardly migrating cells.

Structure	Level	Pattern	Image	Note
TS26: cerebellum external granule cell layer	Weak	Regionally restricted	4D , 4F , 4F' , 4E	Expression is in the posterior external granule layer and in inwardly migrating cells.

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Present	Regionally restricted	4H , 4G , 4I	Expression is in the medial nuclei.

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Present	Regionally restricted	4J , 4K , 4L	Expression is in the medial nuclei.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted		Expression is in the majority of Purkinje cells in all lobules of the vermis and in most of the Purkinje cells in the hemispheres.
TS28: cerebellum vermis lobule	Present	Regionally restricted		Expression is in the majority of Purkinje cells in all lobules of the vermis and in most of the Purkinje cells in the hemispheres.

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Absent