Gene Expression Data
Assay Details
Assay
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Reference:
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J:86960
Darling DS, et al., Expression of Zfhep/deltaEF1 protein in palate, neural progenitors, and differentiated neurons. Gene Expr Patterns. 2003 Dec;3(6):709-17
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5905169
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Antibody:
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clone 40.2D6
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Results
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Specimen
5E/F:
embryonic day 12.5
(more )
Note: Double labeled: red -Isl1; green - Zeb1 (assay MGI:5905162).
Specimen
5E/F:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
|
Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red -Isl1; green - Zeb1 (assay MGI:5905162).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: pons |
Present |
Regionally restricted |
5F
,
5E
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Expression in trigeminal nucleus (Vn) of pons.
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Specimen
5H/I:
embryonic day 12.5
(more )
Note: Double labeled: red -Isl1; green - Zeb1 (assay MGI:5905162).
Specimen
5H/I:
(close )
Genetic Background:
|
Not Specified
|
Age:
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embryonic day 12.5
|
Sex:
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Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red -Isl1; green - Zeb1 (assay MGI:5905162).
|
Specimen
5K:
embryonic day 12.5
(more )
Note: Double labeled: red -Isl1; green - Zeb1 (assay MGI:5905162).
Specimen
5K:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red -Isl1; green - Zeb1 (assay MGI:5905162).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: pons |
Present |
Regionally restricted |
5K
|
Expression in stream of cells from restricted region of basal plate at lateral edge of pons.
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Specimen
5L:
embryonic day 12.5
(more )
Note: Double labeled: red -Isl1; green - Zeb1 (assay MGI:5905162).
Specimen
5L:
(close )
Genetic Background:
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Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red -Isl1; green - Zeb1 (assay MGI:5905162).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: pons |
Present |
Regionally restricted |
5L
|
Expression in migrating cells as they differentiate.
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Specimen
5N/O:
embryonic day 12.5
(more )
Note: Double labeled: red -Isl1; green - Zeb1 (assay MGI:5905162).
Specimen
5N/O:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red -Isl1; green - Zeb1 (assay MGI:5905162).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: pons |
Present |
Regionally restricted |
5O
,
5N
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Expression in trigeminal motor nucleus.
|
Specimen
6G/H:
embryonic day 12.5
(more )
Note: Double labeled: red -Isl1; green - Zeb1 (assay MGI:5905162).
Specimen
6G/H:
(close )
Genetic Background:
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Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red -Isl1; green - Zeb1 (assay MGI:5905162).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cranial ganglion |
Present |
Regionally restricted |
6H
,
6G
|
Expression in X and XI ganglia complex.
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|