Probe:	Apobec1 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS22: mesenchymal layer of pelvic urethra	Absent		4G
TS22: urethra epithelium	Absent		4G

Structure	Level	Pattern	Image	Note
TS25: epithelium of pelvic urethra of male	Absent		4H
TS25: mesenchymal layer of pelvic urethra of male	Absent		4H

Structure	Level	Pattern	Image	Note
TS28: epithelium of pelvic urethra of male	Absent		4I
TS28: mesenchymal layer of pelvic urethra of male	Absent		4I

Structure	Level	Pattern	Image	Note
TS22: lower urogenital tract	Ambiguous		4J	There were no noteworthy sex differences in staining within the lower urinary tract.

Structure	Level	Pattern	Image	Note
TS25: lower urogenital tract of female	Ambiguous		4K	There were no noteworthy sex differences in staining within the lower urinary tract.

Structure	Level	Pattern	Image	Note
TS28: lower urogenital tract of female	Ambiguous		4L	There were no noteworthy sex differences in staining within the lower urinary tract.

Structure	Level	Pattern	Image	Note
TS22: epithelium of pelvic urethra	Absent
TS22: pelvic urethra	Absent			There was no staining in the lamina propria, the muscularis mucosa, the submucosa, or the muscularis propria.
TS22: mesenchyme derived from neural crest	Absent			No staining was detected in the neural crest derivatives of the pelvic urethra.
TS22: developing vasculature of pelvic urethra	Absent
TS22: epithelium of nephric duct of male	Absent
TS22: mesenchyme of nephric duct of male	Absent

Structure	Level	Pattern	Image	Note
TS22: epithelium of pelvic urethra	Absent
TS22: pelvic urethra	Absent			There was no staining in the lamina propria, the muscularis mucosa, the submucosa, or the muscularis propria.
TS22: mesenchyme derived from neural crest	Absent			No staining was detected in the neural crest derivatives of the pelvic urethra.
TS22: developing vasculature of pelvic urethra	Absent
TS22: epithelium of paramesonephric duct of female	Absent
TS22: mesenchyme of paramesonephric duct of female	Weak

Structure	Level	Pattern	Image	Note
TS25: basal cell layer of pelvic urethra of male	Absent
TS25: epithelium of pelvic urethra of male	Absent
TS25: intermediate cell layer of pelvic urethra of male	Absent
TS25: lamina propria of pelvic urethra of male	Absent
TS25: muscle layer of pelvic urethra of male	Absent
TS25: muscularis mucosa of pelvic urethra of male	Absent
TS25: submucosa of pelvic urethra of male	Absent
TS25: superficial cell layer of pelvic urethra of male	Absent
TS25: mesenchymal pad of pelvic urethra of male	Absent
TS25: vasculature of pelvic urethra of male	Absent
TS25: presumptive neural crest derivative of pelvic urethra of male	Absent
TS25: epithelium of seminal vesicle	Absent
TS25: muscle of seminal vesicle	Absent
TS25: vasculature of seminal vesicle	Absent
TS25: seminal vesicle	Absent			No staining was detected in the mesenchymal layer or the adventitia of the seminal vesicle.
TS25: epithelium of ejaculatory duct	Absent
TS25: muscle of ejaculatory duct	Absent
TS25: vasculature of ejaculatory duct	Absent
TS25: ejaculatory duct	Absent			No staining was detected in the mesenchymal layer of the ejaculatory duct.
TS25: peri-prostatic mesenchyme	Absent
TS25: prostate epithelium	Absent

Structure	Level	Pattern	Image	Note
TS25: basal cell layer of pelvic urethra of female	Absent
TS25: epithelium of pelvic urethra of female	Absent
TS25: intermediate cell layer of pelvic urethra of female	Absent
TS25: superficial cell layer of pelvic urethra of female	Absent
TS25: lamina propria of pelvic urethra of female	Absent
TS25: muscle layer of pelvic urethra of female	Absent
TS25: muscularis mucosa of pelvic urethra of female	Absent
TS25: submucosa of pelvic urethra of female	Absent
TS25: mesenchymal pad of pelvic urethra of female	Absent
TS25: presumptive neural crest derivative of pelvic urethra of female	Absent
TS25: vasculature of pelvic urethra of female	Absent
TS25: adventitia of upper vagina	Absent
TS25: epithelium of upper vagina	Absent
TS25: muscle of upper vagina	Absent
TS25: vasculature of upper vagina	Absent
TS25: upper vagina	Absent			No staining was detected in the mesenchymal layer of the upper vagina.
TS25: adventitia of sinovaginal bulb	Absent
TS25: epithelium of sinovaginal bulb	Absent
TS25: muscle of sinovaginal bulb	Absent
TS25: vasculature of sinovaginal bulb	Absent
TS25: sinovaginal bulb	Absent			No staining was detected in the mesenchymal layer of the lower vagina.

Structure	Level	Pattern	Image	Note
TS28: basal cell layer of pelvic urethra of male	Absent
TS28: epithelium of pelvic urethra of male	Absent
TS28: intermediate cell layer of pelvic urethra of male	Absent
TS28: superficial cell layer of pelvic urethra of male	Absent
TS28: lamina propria of pelvic urethra of male	Absent
TS28: muscle layer of pelvic urethra of male	Absent
TS28: muscularis mucosa of pelvic urethra of male	Absent
TS28: submucosa of pelvic urethra of male	Absent
TS28: mesenchymal pad of pelvic urethra of male	Absent
TS28: presumptive neural crest derivative of pelvic urethra of male	Absent
TS28: vasculature of pelvic urethra of male	Absent
TS28: epithelium of seminal vesicle	Absent
TS28: vasculature of seminal vesicle	Absent
TS28: muscle of seminal vesicle	Weak
TS28: seminal vesicle	Weak	Regionally restricted		Staining was low in the mesenchymal layer and the adventitia of the seminal vesicle.
TS28: epithelium of ejaculatory duct	Absent
TS28: muscle of ejaculatory duct	Absent
TS28: vasculature of ejaculatory duct	Absent
TS28: ejaculatory duct	Absent			No staining was detected in the mesenchymal layer of the ejaculatory duct.
TS28: peri-prostatic mesenchyme	Absent
TS28: prostate epithelium	Absent

Structure	Level	Pattern	Image	Note
TS28: basal cell layer of pelvic urethra of female	Weak
TS28: epithelium of pelvic urethra of female	Weak
TS28: intermediate cell layer of pelvic urethra of female	Weak
TS28: superficial cell layer of pelvic urethra of female	Weak
TS28: lamina propria of pelvic urethra of female	Absent
TS28: muscle layer of pelvic urethra of female	Absent
TS28: muscularis mucosa of pelvic urethra of female	Absent
TS28: submucosa of pelvic urethra of female	Weak
TS28: mesenchymal pad of pelvic urethra of female	Absent
TS28: presumptive neural crest derivative of pelvic urethra of female	Absent
TS28: vasculature of pelvic urethra of female	Absent
TS28: epithelium of vagina	Moderate
TS28: adventitia of vagina	Absent
TS28: muscle of vagina	Absent
TS28: vasculature of vagina	Absent
TS28: vagina	Moderate	Regionally restricted		Staining was moderate in the epithelial layer and not detected in the mesenchymal layer of the upper vagina.