Probe:	Aicda cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS22: mesenchymal layer of pelvic urethra	Present		4A
TS22: urethra epithelium	Present		4A

Structure	Level	Pattern	Image	Note
TS25: epithelium of pelvic urethra of male	Weak		4B	Expression gradually decreases from E14.5.
TS25: mesenchymal layer of pelvic urethra of male	Weak		4B	Expression gradually decreases from E14.5.

Structure	Level	Pattern	Image	Note
TS28: epithelium of pelvic urethra of male	Ambiguous		4C	Expression gradually decreases from E14.5 until at least P5.
TS28: mesenchymal layer of pelvic urethra of male	Ambiguous		4C	Expression gradually decreases from E14.5 until at least P5.

Structure	Level	Pattern	Image	Note
TS22: mesenchymal layer of pelvic urethra	Present		4D
TS22: urethra epithelium	Present		4D

Structure	Level	Pattern	Image	Note
TS25: epithelium of pelvic urethra of female	Present		4E	Expression gradually decreases from E14.5.
TS25: mesenchymal layer of pelvic urethra of female	Present		4E	Expression gradually decreases from E14.5.

Structure	Level	Pattern	Image	Note
TS28: epithelium of pelvic urethra of female	Ambiguous		4F	Expression gradually decreases from E14.5 until at least P5.
TS28: mesenchymal layer of pelvic urethra of female	Ambiguous		4F	Expression gradually decreases from E14.5 until at least P5.

Structure	Level	Pattern	Image	Note
TS22: epithelium of pelvic urethra	Moderate
TS22: pelvic urethra	Moderate	Regionally restricted		Medium expression was noted in lamina propria, muscularis mucosa, and muscularis propria; low expression was noted in the submucosa. Staining was not detected in neural crest derivatives.
TS22: developing vasculature of pelvic urethra	Absent
TS22: epithelium of nephric duct of male	Weak
TS22: mesenchyme of nephric duct of male	Moderate

Structure	Level	Pattern	Image	Note
TS22: epithelium of pelvic urethra	Moderate
TS22: pelvic urethra	Moderate	Regionally restricted		Medium expression was noted in lamina propria, muscularis mucosa, and muscularis propria; low expression was noted in the submucosa. Staining was not detected in neural crest derivatives.
TS22: developing vasculature of pelvic urethra	Absent
TS22: epithelium of paramesonephric duct of female	Weak
TS22: mesenchyme of paramesonephric duct of female	Moderate

Structure	Level	Pattern	Image	Note
TS25: epithelium of pelvic urethra of male	Moderate
TS25: superficial cell layer of pelvic urethra of male	Moderate
TS25: intermediate cell layer of pelvic urethra of male	Moderate
TS25: basal cell layer of pelvic urethra of male	Weak
TS25: lamina propria of pelvic urethra of male	Absent
TS25: muscularis mucosa of pelvic urethra of male	Absent
TS25: submucosa of pelvic urethra of male	Moderate
TS25: muscle layer of pelvic urethra of male	Absent
TS25: mesenchymal pad of pelvic urethra of male	Moderate
TS25: presumptive neural crest derivative of pelvic urethra of male	Absent
TS25: vasculature of pelvic urethra of male	Absent
TS25: epithelium of seminal vesicle	Moderate
TS25: seminal vesicle	Weak	Regionally restricted		Low abundance expression was noted in the mesenchymal layer of the seminal vesicle. Expression was not detected in the adventitia of the seminal vesicle.
TS25: muscle of seminal vesicle	Absent
TS25: vasculature of seminal vesicle	Absent
TS25: epithelium of ejaculatory duct	Moderate
TS25: ejaculatory duct	Weak	Regionally restricted		Low abundance was noted in the mesenchymal layer of the ejaculatory duct.
TS25: muscle of ejaculatory duct	Absent
TS25: vasculature of ejaculatory duct	Absent
TS25: peri-prostatic mesenchyme	Moderate
TS25: prostate epithelium	Moderate

Structure	Level	Pattern	Image	Note
TS25: epithelium of pelvic urethra of female	Moderate
TS25: intermediate cell layer of pelvic urethra of female	Moderate
TS25: superficial cell layer of pelvic urethra of female	Moderate
TS25: basal cell layer of pelvic urethra of female	Weak
TS25: mesenchymal pad of pelvic urethra of female	Weak
TS25: submucosa of pelvic urethra of female	Weak
TS25: lamina propria of pelvic urethra of female	Absent
TS25: muscle layer of pelvic urethra of female	Absent
TS25: muscularis mucosa of pelvic urethra of female	Absent
TS25: presumptive neural crest derivative of pelvic urethra of female	Absent
TS25: vasculature of pelvic urethra of female	Absent
TS25: epithelium of upper vagina	Moderate
TS25: upper vagina	Weak	Regionally restricted		Low abundance was noted in the mesenchymal layer of the upper vagina.
TS25: muscle of upper vagina	Weak
TS25: adventitia of upper vagina	Absent
TS25: vasculature of upper vagina	Absent
TS25: epithelium of sinovaginal bulb	Moderate
TS25: sinovaginal bulb	Weak	Regionally restricted		Low abundance was noted in the mesenchymal layer of the lower vagina.
TS25: muscle of sinovaginal bulb	Weak
TS25: adventitia of sinovaginal bulb	Absent
TS25: vasculature of sinovaginal bulb	Absent

Structure	Level	Pattern	Image	Note
TS28: epithelium of pelvic urethra of male	Weak
TS28: intermediate cell layer of pelvic urethra of male	Weak
TS28: lamina propria of pelvic urethra of male	Weak
TS28: submucosa of pelvic urethra of male	Weak
TS28: superficial cell layer of pelvic urethra of male	Weak
TS28: basal cell layer of pelvic urethra of male	Absent
TS28: muscle layer of pelvic urethra of male	Absent
TS28: muscularis mucosa of pelvic urethra of male	Absent
TS28: vasculature of pelvic urethra of male	Absent
TS28: mesenchymal pad of pelvic urethra of male	Absent
TS28: presumptive neural crest derivative of pelvic urethra of male	Moderate
TS28: epithelium of seminal vesicle	Moderate
TS28: muscle of seminal vesicle	Moderate
TS28: seminal vesicle	Moderate	Regionally restricted		Medium expression was detected in the seminal vesicle mesenchymal layer. There was no staining in the adventitia.
TS28: vasculature of seminal vesicle	Absent
TS28: epithelium of ejaculatory duct	Moderate
TS28: muscle of ejaculatory duct	Moderate
TS28: ejaculatory duct	Moderate	Regionally restricted		Medium expression was detected in the ejaculatory duct mesenchymal layer.
TS28: vasculature of ejaculatory duct	Absent
TS28: prostate epithelium	Absent
TS28: peri-prostatic mesenchyme	Weak

Structure	Level	Pattern	Image	Note
TS28: basal cell layer of pelvic urethra of female	Moderate
TS28: epithelium of pelvic urethra of female	Moderate
TS28: intermediate cell layer of pelvic urethra of female	Moderate
TS28: superficial cell layer of pelvic urethra of female	Moderate
TS28: lamina propria of pelvic urethra of female	Absent
TS28: muscle layer of pelvic urethra of female	Absent
TS28: muscularis mucosa of pelvic urethra of female	Absent
TS28: submucosa of pelvic urethra of female	Absent
TS28: vasculature of pelvic urethra of female	Absent
TS28: mesenchymal pad of pelvic urethra of female	Absent
TS28: presumptive neural crest derivative of pelvic urethra of female	Absent
TS28: epithelium of vagina	Moderate
TS28: vagina	Weak	Regionally restricted		The staining was low in upper vagina mesenchymal layer.
TS28: adventitia of vagina	Absent
TS28: muscle of vagina	Absent
TS28: vasculature of vagina	Absent