Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:214712
Le Bras B, et al., In vivo assembly of the axon initial segment in motor neurons. Brain Struct Funct. 2014 Jul;219(4):1433-50
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5828213
|
|
Gene symbol:
|
Ank3
|
|
Gene name:
|
ankyrin 3, epithelial
|
|
|
Results
|
Specimen
6a1:
embryonic day 9.0
(more  )
Specimen
6a1:
(close  )
|
Genetic Background:
|
OF1
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
6b1:
embryonic day 9.25
(more )
Note: Double labeled: green - Ank3; red - Nfasc (assay MGI:5828263).
Specimen
6b1:
(close )
|
Genetic Background:
|
OF1
|
|
Age:
|
embryonic day 9.25
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Ank3; red - Nfasc (assay MGI:5828263).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube lateral wall |
Present |
Regionally restricted |
6b1
,
6b3
|
Expression is in a few motor neurons and along their axons extending out of the spinal cord.
|
Specimen
6c1:
embryonic day 10.5
(more )
Note: Double labeled: green - Ank3; red - Nfasc (assay MGI:5828263).
Specimen
6c1:
(close )
|
Genetic Background:
|
OF1
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Ank3; red - Nfasc (assay MGI:5828263).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube lateral wall |
Present |
Regionally restricted |
6c3
,
6c1
|
Expression is in motor neurons and along their axons extending out of the spinal cord.
|
Specimen
6d1:
embryonic day 11.5
(more )
Note: Double labeled: green - Ank3; red - Nfasc (assay MGI:5828263).
Specimen
6d1:
(close )
|
Genetic Background:
|
OF1
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Ank3; red - Nfasc (assay MGI:5828263).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural tube lateral wall |
Present |
Regionally restricted |
6d3
,
6d1
|
Expression is in the axons of motor neurons with a distal to proximal restriction.
|
Specimen
6e1:
embryonic day 13.5
(more )
Note: Double labeled: green - Ank3; red - Nfasc (assay MGI:5828263).
Specimen
6e1:
(close )
|
Genetic Background:
|
OF1
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Ank3; red - Nfasc (assay MGI:5828263).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: basal columns |
Present |
Regionally restricted |
6e3
,
6e1
|
Expression is in the proximal axonal segments of motor neurons in the spinal cord.
|
Specimen
6f1:
embryonic day 12.5
(more )
Specimen
6f1:
(close )
|
Genetic Background:
|
OF1
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: basal columns |
Present |
Regionally restricted |
6f1
|
Expression is in the axons of motor neurons with a distal to proximal restriction.
|
Specimen
6f2:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Nfasctm1.1Brp/Nfasctm1.1Brp
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: basal columns |
Present |
Regionally restricted |
6f2
|
Expression in this mutant is the same as in wild-type.
|
Specimen
6g1:
embryonic day 13.5
(more )
Specimen
6g1:
(close )
|
Genetic Background:
|
OF1
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: ventral grey horn |
Present |
Regionally restricted |
6g1
|
Expression is in the proximal axonal segments of motor neurons in the spinal cord.
|
Specimen
6g2:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Nfasctm1.1Brp/Nfasctm1.1Brp
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: ventral grey horn |
Present |
Regionally restricted |
6g2
|
Expression in this mutant is the same as in wild-type.
|
Specimen
S1b:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Nfasctm1.1Brp/Nfasctm1.1Brp
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S1d2:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Nfasctm1.1Brp/Nfasctm1.1Brp
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - Ank3 (guinea pig antibody); blue - Ank3 (rabbit antibody; assay MGI:5828210); green - Ank3 (mouse antibody; assay MGI:5828294).
|
|
Analysis Tools