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Gene Expression Data
Assay Details
Assay
Reference: J:189751 Hara T, et al., Identification of IL-7-producing cells in primary and secondary lymphoid organs using IL-7-GFP knock-in mice. J Immunol. 2012 Aug 15;189(4):1577-84
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5796369
Gene symbol: Il7
Gene name: interleukin 7
Reporter: GFP
Antibody: anti-GFP
Results
Specimen 2B: postnatal week 7; Il7tm1.1Iku/Il7+ (more )
Note: Double labeled: red - Il7 reporter; blue - ER-TR7.
Structure Level Pattern Image Note
TS28: thymus Present 2B GFP ER-TR7 , 2B GFP
Specimen 2Da: postnatal week 7; Il7tm1.1Iku/Il7+ (more )
Note: Double labeled: red - Il7 reporter; blue - Vcam1 (assay MGI:5796513).
Structure Level Pattern Image Note
TS28: bone marrow Present 2D GFP VCAM1 GFP-KI , 2D GFP
Specimen 2Db: postnatal week 7; Il7tm1.1Iku/Il7+ (more )
Note: Double labeled: red - Il7 reporter; blue - Vcam1 (assay MGI:5796513).
Structure Level Pattern Image Note
TS28: bone marrow Present 2D b Includes expression near the endosteum.
Specimen 4Aa: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Triple labeled: red - Il7 reporter; green - Cd3e (assay MGI:5803998); blue - Ptprc (assay MGI:5796514).
Structure Level Pattern Image Note
TS28: spleen Present Regionally restricted 4Aa GFP , 4Aa GFP CD3 B220 GFP-KI Expression is in stromal cells within the periarteriolar lymphoid sheath of the spleen.
Specimen 4Ab: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Double labeled: red - Il7 reporter; green - ER-TR7.
Structure Level Pattern Image Note
TS28: spleen Present Regionally restricted 4Ab GFP , 4Ab GFP ER-TR7 Expression is in flibroblastic reticular cells surrounding the central arterioles.
Specimen 4Ac: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Triple labeled: red - Il7 reporter; green - Pecam1 (assay MGI:5804000); blue - Cd3e (assay MGI:5803998).
Structure Level Pattern Image Note
TS28: spleen Present Regionally restricted 4Ac Expression is in flibroblastic reticular cells surrounding the central arterioles.
Specimen 4Ba: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Triple labeled: red - Il7 reporter; green - ER-TR7; blue - Cd3e (assay MGI:5803998).
Structure Level Pattern Image Note
TS28: lymph node cortex Strong Regionally restricted 4Ba GFP ER-TR7 CD3 GFP-KI , 4Ba GFP Expression is in the cortical ridge.
Specimen 4Bb: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Triple labeled: red - Il7 reporter; green - ER-TR7; blue - Cd3e (assay MGI:5803998).
Structure Level Pattern Image Note
TS28: lymph node Present Regionally restricted 4Bb GFP , 4Bb GFP ER-TR7 CD3 Sparse distribution in the center of the paracortex.
Specimen 4C: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Triple labeled: red - Il7 reporter; green - ER-TR7; blue - Cd3e (assay MGI:5803998).
Structure Level Pattern Image Note
TS28: mesenteric lymph node Present 4C GFP , 4C GFP ER-TR7 CD3
Specimen 5Aa SI: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Double labeled: blue - Il7 reporter; red - Epcam (assay MGI:5796512).
Structure Level Pattern Image Note
TS28: small intestine epithelium Present Non-Uniform 5Aa SI GFP , 5Aa SI GFP EpCAM GFP-KI
Specimen 5Aa LI: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Double labeled: blue - Il7 reporter; red - Epcam (assay MGI:5796512).
Structure Level Pattern Image Note
TS28: large intestine epithelium Present Non-Uniform 5Aa LI GFP , 5Aa LI GFP EpCAM GFP-KI
Specimen 5Ab SI: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Double labeled: blue - Il7 reporter; red - Lyve1 (assay MGI:5804001).
Structure Level Pattern Image Note
TS28: small intestine epithelium Present Non-Uniform 5Ab SI GFP Lyve1 GFP-KI , 5Ab SI GFP
TS28: lymphatic vessel endothelium Present Regionally restricted 5Ab SI GFP Lyve1 GFP-KI , 5Ab SI GFP Expression is in intestinal lymphatic endothelium.
Specimen 5Ab LI: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Double labeled: blue - Il7 reporter; red - Lyve1 (assay MGI:5804001).
Structure Level Pattern Image Note
TS28: large intestine epithelium Present Non-Uniform 5Ab LI GFP , 5Ab LI GFP Lyve1 GFP-KI
TS28: lymphatic vessel endothelium Present Regionally restricted 5Ab LI GFP , 5Ab LI GFP Lyve1 GFP-KI Expression is in intestinal lymphatic endothelium.
Specimen 5C: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Triple labeled: red - Il7 reporter; green - ER-TR7; blue - Cd3e (assay MGI:5803998).
Structure Level Pattern Image Note
TS28: Peyer's patch Present 5C GFP ER-TR7 CD3 , 5C GFP
Specimen 5D: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Triple labeled: red - Il7 reporter; green - ER-TR7; blue - Cd3e (assay MGI:5803998).
Structure Level Pattern Image Note
TS28: lymph node follicle Present Scattered 5D GFP ER-TR7 , 5D GFP ER-TR7 CD3
Specimen 6B Spleen: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Double labeled: red - Il7 reporter; green - Cd3e (assay MGI:5803998).
Structure Level Pattern Image Note
TS28: spleen Present 6B GFP CD11c Mac1 control , 6B GFP CD3 control
Specimen 6B SI: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Triple labeled: blue- Il7 reporter; green - Itgax (assay MGI:5804010); red - Itgam (assay MGI:5804009).
Structure Level Pattern Image Note
TS28: small intestine Present 6B GFP CD11c Mac1 control
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory