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Gene Expression Data
Assay Details
Assay
Reference: J:189751 Hara T, et al., Identification of IL-7-producing cells in primary and secondary lymphoid organs using IL-7-GFP knock-in mice. J Immunol. 2012 Aug 15;189(4):1577-84
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5796358
Gene symbol: Il7
Gene name: interleukin 7
Reporter: GFP
Antibody: anti-GFP (#A21311)
Detection system: Alexa Fluor 488
Results
Specimen 2A left column: postnatal week 7; Il7tm1.1Iku/Il7+ (more )
Note: Double labeled: green - Il7 reporter; blue - Enpep (assay MGI:5796395).
Structure Level Pattern Image Note
TS28: thymus Present 2A middle GFP , 2A left GFP Ly51 , 2A left GFP , 2A left HM Expression is in thymic stromal cells.
Specimen 2A middle column: postnatal week 7; Il7tm1.1Iku/Il7+ (more )
Note: Double labeled: green - Il7 reporter; blue - Enpep (assay MGI:5796395).
Structure Level Pattern Image Note
TS28: thymus cortex Present 2A middle GFP Ly51 , 2A middle GFP , 2A middle HM
Specimen 2A right column: postnatal week 7; Il7tm1.1Iku/Il7+ (more )
Note: Double labeled: green - Il7 reporter; blue - Enpep (assay MGI:5796395).
Structure Level Pattern Image Note
TS28: thymus cortex Present 2A right GFP , 2A right GFP Ly51 , 2A right HM
TS28: thymus medullary core Present 2A right GFP , 2A right GFP Ly51 , 2A right HM
Specimen 2C top: embryonic day 16.5; Il7tm1.1Iku/Il7+ (more )
Structure Level Pattern Image Note
TS24: thymus Present 2C GFP
Specimen 2C bottom: embryonic day 16.5; Il7tm1.1Iku/Il7+ (more )
Note: Double labeled: green - Il7 reporter; red - Epcam (assay MGI:5796512).
Structure Level Pattern Image Note
TS24: thymus Present 2C GFP EpCAM GFP-KI
Specimen 4B c KI: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Double labeled: green - Il7 reporter; red - Lyve1 (assay MGI:5804001).
Structure Level Pattern Image Note
TS28: lymph node Present 4Bc GFP , 4Bc GFP Lyve1 , 4Bc HM GFP , 4Bc HM GFP Lyve1
Specimen 4E: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Double labeled: green - Il7 reporter; red - Lyve1 (assay MGI:5804001).
Structure Level Pattern Image Note
TS28: dermis Present 4E IL-7-GFP-KI
TS28: ear skin Present 4E IL-7-GFP-KI
Specimen 6A LI top: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Triple labeled: green - Il7 reporter; red - Itgam (assay MGI:5804009); blue - Cd4 (assay MGI:5804013).
Structure Level Pattern Image Note
TS28: large intestine Present 6A GFP CD4 Mac1 control
Specimen 6A LI bottom: postnatal; Il7tm1.1Iku/Il7+ (more )
Note: Triple labeled: green - Il7 reporter; red - Itgax (assay MGI:5804010); blue - Cd4 (assay MGI:5804013).
Structure Level Pattern Image Note
TS28: large intestine Present 6A GFP CD4 CD11c control
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory