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Gene Expression Data
Assay Details
Assay
Reference: J:22553 Guz Y, et al., Expression of murine STF-1, a putative insulin gene transcription factor, in beta cells of pancreas, duodenal epithelium and pancreatic exocrine and endocrine progenitors during ontogeny. Development. 1995 Jan;121(1):11-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5763791
Gene symbol: Pdx1
Gene name: pancreatic and duodenal homeobox 1
Antibody: anti-STF-1
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: The authors state that identical results were obtained with the XIH box 8 antibody MGI:5763801.
Results
Specimen 1A: embryonic day 8.5 (more )
Note: 11-13 somites
Structure Level Pattern Image Note
TS13: gut Present Regionally restricted 1A Expression is in the endodermal layer in the dorsal wall.

Specimen 1B: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: foregut-midgut junction Present Regionally restricted 1B Expression is in nuclei in the the dorsal pancreas and ventral pancreatic primordia.

Specimen 1C: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: pancreatic duct Present Regionally restricted 1C Expression is throughout the pancreatic duct epithelium and in small clusters.

Specimen 1D: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: pancreatic duct Present Regionally restricted 1D Expression is throughout the pancreatic duct epithelium and in small clusters.

Specimen 1E: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: islets of Langerhans Present 1E
TS25: pancreatic duct Trace 1E

Specimen 1F: postnatal adult (more )
Structure Level Pattern Image Note
TS28: islets of Langerhans Present 1F
TS28: exocrine pancreas Absent 1F

Specimen 2A: embryonic day 9.5 (more )
Note: 13-20 somites
Structure Level Pattern Image Note
TS15: gut Present Regionally restricted 2A Expression is in a transverse band of endoderm along the dorsal, lateral and ventral regions of the presumptive duodenum and in cells forming the dorsal pancreatic primordium.

Specimen 2B: postnatal adult (more )
Structure Level Pattern Image Note
TS28: duodenum epithelium Present Regionally restricted 2B Expression is in the epithelium that lines the villi and is absent in cells in the crypt.

Specimen 3A: embryonic day 9.5 (more )
Note: Double labeled: brown - Pdx1; blue - Gcg (assay MGI:5763804).
Structure Level Pattern Image Note
TS15: foregut-midgut junction Present Regionally restricted 3A Expression is in the pancreatic primordium.

Specimen 3B: embryonic day 13.5 (more )
Note: Double labeled: brown - Pdx1; blue - Gcg (assay MGI:5763804).
Structure Level Pattern Image Note
TS21: pancreas Present 3B

Specimen 3C: embryonic day 14.5 (more )
Note: Double labeled: brown - Pdx1; blue - Ins1 (assay MGI:5763806) and Ins2 (assay MGI:5763808).
Structure Level Pattern Image Note
TS22: pancreas Present 3C

Specimen 3D: embryonic day 14.5 (more )
Note: Double labeled: brown - Pdx1; blue - Sst (assay MGI:5763813).
Structure Level Pattern Image Note
TS22: pancreas Present 3D

Specimen 3E: postnatal day 1 (more )
Note: Double labeled: brown - Pdx1; blue - Ppy (assay MGI:5763811).
Structure Level Pattern Image Note
TS27: pancreas Present 3E

Specimen 3F: postnatal adult (more )
Note: Double labeled: brown - Pdx1; blue - Ppy (assay MGI:5763811).
Structure Level Pattern Image Note
TS28: pancreas Present 3F

Specimen 4A: embryonic day 15.5 (more )
Note: Double labeled: brown - Pdx1; blue - Amy (assay MGI:5763815).
Structure Level Pattern Image Note
TS23: pancreas Present 4A

Specimen 4B: embryonic day 15.5 (more )
Note: Double labeled: brown - Pdx1; blue - Amy (assay MGI:5763815).
Structure Level Pattern Image Note
TS23: pancreas Present 4B

Specimen 4C: embryonic day 17.5 (more )
Note: Double labeled: brown - Pdx1; blue - Amy (assay MGI:5763815).
Structure Level Pattern Image Note
TS25: pancreas Present 4C

Specimen 4D: postnatal adult (more )
Note: Double labeled: brown - Pdx1; blue - Amy (assay MGI:5763815).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present 4D
TS28: exocrine pancreas Absent 4D

Specimen not shown E8: embryonic day 8.0 (more )
Note: 5-7 somites
Structure Level Pattern Image Note
TS12: gut Absent

Specimen not shown E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: pancreas Present Regionally restricted Expression is in newly formed islets.

Specimen Table 1 E9.5: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: gut Present Expression is in the pancreatic primordia.

Specimen Table 1 E13.5: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: pancreas Present

Specimen Table 1 E14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: pancreas Present

Specimen Table 1 E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: pancreas Present

Specimen Table 1 P1: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: pancreas Present


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last database update
05/28/2024
MGI 6.13
The Jackson Laboratory