Gata3 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:85304 Kaufman CK, et al., GATA-3: an unexpected regulator of cell lineage determination in skin. Genes Dev. 2003 Sep 1;17(17):2108-22
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:5760379
Gene symbol:	Gata3
Gene name:	GATA binding protein 3

Reporter:	lacZ
Antibody:	anti-B-galactosidase (rabbit)
Detection system:	Secondary antibody coupled to fluorescein

Results

Specimen 2C: postnatal day 6; Gata3^tm1Gsv/Gata3⁺ (more

)
Note: Post-fixed with 4% paraformaldehyde. Double labeled: green - Gata3 reporter; red - pan cytokeratin.

Structure	Level	Pattern	Image	Note
TS28: hair inner root sheath	Present	Regionally restricted	2C	Expressed in two cell layers, which were external to the hair shaft cuticle and cortex/precortex.

Specimen 2D: postnatal day 6; Gata3^tm1Gsv/Gata3⁺ (more

)
Note: Post-fixed with 4% paraformaldehyde. Double labeled: green - Gata3 reporter; red - Tchh (assay MGI:5760380).

Structure	Level	Pattern	Image	Note
TS28: hair inner root sheath	Present	Regionally restricted	2D	Expressed in two cell layers, which were directly internal to a single layer of medulla cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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