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Gene Expression Data
Assay Details
Assay
Reference: J:137940 Lange UC, et al., Normal germ line establishment in mice carrying a deletion of the Ifitm/Fragilis gene family cluster. Mol Cell Biol. 2008 Aug;28(15):4688-96
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5760375
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct3/4
Detection system: Secondary antibody
Results
Specimen 2F Del+/- Oct4 upper: embryonic day 12.5; Del(7Ifitm5-Ifitm6)1Masu/+ (more )
Note: Triple labeled: green - Oct4 (Pou5f1); red - K9H3me2; blue - DAPI.
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 2F Del+/- Oct4 (upper) , 2F Del+/- merge+DAPI (upper)
Specimen 2F Del-/- Oct4 upper: embryonic day 12.5; Del(7Ifitm5-Ifitm6)1Masu/Del(7Ifitm5-Ifitm6)1Masu (more )
Note: Triple labeled: green - Oct4 (Pou5f1); red - K9H3me2; blue - DAPI.
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 2F Del-/- merge+DAPI (upper) , 2F Del-/- Oct4 (upper)
Specimen 2F Del+/- Oct4 lower: embryonic day 12.5; Del(7Ifitm5-Ifitm6)1Masu/+ (more )
Note: Triple labeled: green - Oct4 (Pou5f1); red - K27H3me3; blue - DAPI.
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 2F Del+/- Oct4 (lower) , 2F Del+/- merge+DAPI (lower)
Specimen 2F Del-/- Oct4 lower: embryonic day 12.5; Del(7Ifitm5-Ifitm6)1Masu/Del(7Ifitm5-Ifitm6)1Masu (more )
Note: Triple labeled: green - Oct4 (Pou5f1); red - K27H3me3; blue - DAPI.
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 2F Del-/- Oct4 (lower) , 2F Del-/- merge+DAPI (lower)
Specimen 4H EGFP/wt Oct4: embryonic day 12.5; Ifitm3tm1Masu/Ifitm3+ (more )
Note: Triple labeled: green - Oct4 (Pou5f1); red - Stella/PGC7 (Dppa3 assay MGI:5760369): blue - DAPI.
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 4H merge+DAPI (upper left) , 4H EGFP/wt Oct4
Specimen 4H EGFP/EGFP Oct4: embryonic day 12.5; Ifitm3tm1Masu/Ifitm3tm1Masu (more )
Note: Triple labeled: green - Oct4 (Pou5f1); red - Stella/PGC7 (Dppa3 assay MGI:5760369): blue - DAPI.
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 4H merge+DAPI (lower right) , 4H EGFP/EGFP Oct4
Specimen S4B Del+/- Oct4: embryonic day 12.5; Del(7Ifitm5-Ifitm6)1Masu/+ (more )
Note: Triple labeled: green - Oct4 (Pou5f1); red - Ifitm3 (assay MGI:5760372): blue - DAPI.
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present S4B Del+/- Oct4 , S4B Del+/- merge+DAPI (upper)
Specimen S4B Del-/- Oct4: embryonic day 12.5; Del(7Ifitm5-Ifitm6)1Masu/Del(7Ifitm5-Ifitm6)1Masu (more )
Note: Triple labeled: green - Oct4 (Pou5f1); red - Ifitm3 (assay MGI:5760372): blue - DAPI.
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present S4B Del-/- merge+DAPI (lower) , S4B Del-/- Oct4
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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