Gene Expression Data
Assay Details
Assay
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Reference:
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J:161846
Westfall JE, et al., Retinal degeneration and failure of photoreceptor outer segment formation in mice with targeted deletion of the Joubert syndrome gene, Ahi1. J Neurosci. 2010 Jun 30;30(26):8759-68
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5751130
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Gene symbol:
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Rho
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Gene name:
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rhodopsin
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Antibody:
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4D2
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Detection system:
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Secondary antibody
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Assay notes:
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Fixative was 1% or 4 % paraformaldehyde.
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Results
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Specimen
3A Ahi1+/+:
embryonic day 18.5
(more )
Specimen
3A Ahi1+/+:
(close )
Genetic Background:
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involves: BALB/cJ * C57BL/6 * C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: neural retina |
Present |
Regionally restricted |
3A Ahi1+/+
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Expression is in some cells of the photoreceptor layer.
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Specimen
3A Ahi1-/-:
(close )
Genetic Background:
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involves: BALB/cJ * C57BL/6 * C57BL/6J
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Mutant Allele(s):
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Ahi1tm1Rujf/Ahi1tm1Rujf
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5A +/+ PN11:
postnatal day 11
(more )
Specimen
5A +/+ PN11:
(close )
Genetic Background:
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involves: BALB/cJ * C57BL/6 * C57BL/6J
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Age:
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postnatal day 11
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5A +/+ PN22:
postnatal day 22
(more )
Specimen
5A +/+ PN22:
(close )
Genetic Background:
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involves: BALB/cJ * C57BL/6 * C57BL/6J
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Age:
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postnatal day 22
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5A +/+ PN42:
postnatal day 42
(more )
Specimen
5A +/+ PN42:
(close )
Genetic Background:
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involves: BALB/cJ * C57BL/6 * C57BL/6J
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Age:
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postnatal day 42
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5A -/- PN11:
(close )
Genetic Background:
|
involves: BALB/cJ * C57BL/6 * C57BL/6J
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Mutant Allele(s):
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Ahi1tm1Rujf/Ahi1tm1Rujf
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Age:
|
postnatal day 11
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5A -/- PN22:
(close )
Genetic Background:
|
involves: BALB/cJ * C57BL/6 * C57BL/6J
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Mutant Allele(s):
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Ahi1tm1Rujf/Ahi1tm1Rujf
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Age:
|
postnatal day 22
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5A -/- PN42:
(close )
Genetic Background:
|
involves: BALB/cJ * C57BL/6 * C57BL/6J
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Mutant Allele(s):
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Ahi1tm1Rujf/Ahi1tm1Rujf
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Age:
|
postnatal day 42
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5B Ahi1+/+:
postnatal day 11
(more )
Specimen
5B Ahi1+/+:
(close )
Genetic Background:
|
involves: BALB/cJ * C57BL/6 * C57BL/6J
|
Age:
|
postnatal day 11
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
Paraformaldehyde
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Embedding:
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Cryosection
|
Specimen
5B Ahi1-/-:
(close )
Genetic Background:
|
involves: BALB/cJ * C57BL/6 * C57BL/6J
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Mutant Allele(s):
|
Ahi1tm1Rujf/Ahi1tm1Rujf
|
Age:
|
postnatal day 11
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6A:
postnatal
(more )
Specimen
6A:
(close )
Genetic Background:
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involves: BALB/cJ * C57BL/6 * C57BL/6J
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6B:
(close )
Genetic Background:
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involves: BALB/cJ * C57BL/6 * C57BL/6J
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Mutant Allele(s):
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Ahi1tm1Rujf/Ahi1tm1Rujf
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
8A Ahi1+/+:
embryonic day 18.5
(more )
Note: Double labeled: red - Rho; green - Rab8a (assay MGI:5751265).
Specimen
8A Ahi1+/+:
(close )
Genetic Background:
|
involves: BALB/cJ * C57BL/6 * C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Rho; green - Rab8a (assay MGI:5751265).
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Specimen
8A Ahi1-/-:
(close )
Genetic Background:
|
involves: BALB/cJ * C57BL/6 * C57BL/6J
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Mutant Allele(s):
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Ahi1tm1Rujf/Ahi1tm1Rujf
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Age:
|
embryonic day 18.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
Paraformaldehyde
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Embedding:
|
Cryosection
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Note:
|
Double labeled: red - Rho; green - Rab8a (assay MGI:5751265).
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