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Gene Expression Data
Assay Details
Assay
Reference: J:147897 Unhavaithaya Y, et al., MILI, a PIWI-interacting RNA-binding protein, is required for germ line stem cell self-renewal and appears to positively regulate translation. J Biol Chem. 2009 Mar 6;284(10):6507-19
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5707857
Gene symbol: Piwil2
Gene name: piwi-like RNA-mediated gene silencing 2
Results
Specimen 3A: postnatal adult (more )
Note: Triple labeled: red - Piwil2; green - laminin; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 3A Specific expression in cytoplasm of spermatogonia and pachytene stage spermatocytes.
TS28: spermatocyte Present Regionally restricted 3A No expression in more differentiated germ cells.
TS28: spermatogonium Present Regionally restricted 3A No expression in more differentiated germ cells.
TS28: spermatid Absent 3A
Specimen 3B: postnatal adult (more )
Note: Triple labeled: red - Piwil2; green - laminin; blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 3B
TS28: spermatocyte Present Regionally restricted 3B Specific expression in cytoplasm of spermatogonia and pachytene stage spermatocytes.
TS28: spermatogonium Present Regionally restricted 3B Specific expression in cytoplasm of spermatogonia and pachytene stage spermatocytes.
TS28: spermatid Absent 3B No expression in more differentiated germ cells.
TS28: Sertoli cell Sertoli cell Absent 3B
Specimen 3C: postnatal adult (more )
Note: Triple labeled: red - Piwil2; green - Tsx (assay MGI:5707858); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS28: seminiferous tubule epithelium Present 3C
TS28: spermatocyte Present 3C
TS28: spermatogonium Present 3C
TS28: Sertoli cell Sertoli cell Absent 3C
Specimen 3D: postnatal adult (more )
Note: Triple labeled: red - Piwil2; green - Ge-1 (Edc4; assay MGI:5707859); blue - DAPI.
Structure Level Pattern Image Note
TS28: germ cell of testis Present Regionally restricted 3D In the germ line cytoplasm, expression enriched in spherical structures.
Specimen 3F/3H: postnatal adult (more )
Note: Triple labeled: red - Piwil2; green - Plzf (Zbtb16; assay MGI:5707860); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatogonium Present 3H , 3F Expression in all spermatogonia.
Specimen S2C: postnatal adult; Piwil2tm1Nkn/Piwil2+ (more )
Note: Triple labeled: red - Piwil2; green - Tsx (assay MGI:5707858); blue - DAPI.
Structure Level Pattern Image Note
TS28: germ cell of testis Present S2C
Specimen S2D: postnatal adult; Piwil2tm1Nkn/Piwil2tm1Nkn (more )
Note: Triple labeled: red - Piwil2; green - Tsx (assay MGI:5707858); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Absent S2D
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory