Gene Expression Data
Assay Details
Assay
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Reference:
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J:220858
Kim JY, et al., Common and distinctive localization patterns of Crumbs polarity complex proteins in the mammalian eye. Gene Expr Patterns. 2015 Jan;17(1):31-7
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5697431
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Gene symbol:
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Pals1
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Gene name:
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protein associated with LIN7 1, MAGUK family member
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Results
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Specimen
2A:
embryonic day 12.5
(more )
Specimen
2A:
(close )
Genetic Background:
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either: C57BL/6J or Swiss Webster
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3A:
embryonic day 13.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
either: C57BL/6J or Swiss Webster
|
Age:
|
embryonic day 13.5
|
Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS21: retina |
Present |
Punctate |
3A
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Widespread punctate staining in the inner retina.
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Specimen
3B:
embryonic day 16.5
(more )
Note: (E,H) are higher magnifications of the boxed areas in (B). Double labeled: red - Pals1; green - Cdh2 (assay MGI:5697432).
Specimen
3B:
(close )
Genetic Background:
|
either: C57BL/6J or Swiss Webster
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Age:
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embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
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optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
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(E,H) are higher magnifications of the boxed areas in (B). Double labeled: red - Pals1; green - Cdh2 (assay MGI:5697432).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: retina |
Present |
Regionally restricted |
3E
,
3B
,
3E2
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Expression is at the apical surface of the retina.
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TS24: retina nuclear layer |
Present |
Regionally restricted |
3H2
,
3B
,
3H
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Expression is in the cytoplasm of retinal cells as well as in apical junctions of most retinal cells in the outer and inner neuroblastic layers.
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Specimen
3C:
embryonic day 19.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
either: C57BL/6J or Swiss Webster
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Age:
|
embryonic day 19.5
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Sex:
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Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: retina |
Present |
Regionally restricted |
3C
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Expression is at the apical surface of the retinal epithelium.
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Specimen
3D:
postnatal day 21
(more )
Specimen
3D:
(close )
Genetic Background:
|
either: C57BL/6J or Swiss Webster
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Age:
|
postnatal day 21
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina |
Present |
Regionally restricted |
3D
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Expression is at the apical surface of the retinal epithelium.
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Specimen
3F:
embryonic day 16.5
(more )
Note: Double labeled: red - Pals1; green - Prkci (assay MGI:5697433) and Prkcz (assay MGI:5697519).
Specimen
3F:
(close )
Genetic Background:
|
either: C57BL/6J or Swiss Webster
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Age:
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embryonic day 16.5
|
Sex:
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Not Specified
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Type:
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optical section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Pals1; green - Prkci (assay MGI:5697433) and Prkcz (assay MGI:5697519).
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Specimen
3G:
postnatal day 2
(more )
Note: Double labeled: red - Pals1; green - Ezr (assay MGI:5697434).
Specimen
3G:
(close )
Genetic Background:
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either: C57BL/6J or Swiss Webster
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Age:
|
postnatal day 2
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Note:
|
Double labeled: red - Pals1; green - Ezr (assay MGI:5697434).
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Specimen
4A:
embryonic day 14.5
(more )
Note: (A1,C1) are higher magnifications of the boxed areaa in (A,C). Double labeled: red - Pals1; green - Golga2 (assay MGI:5697453).
Specimen
4A:
(close )
Genetic Background:
|
either: C57BL/6J or Swiss Webster
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
(A1,C1) are higher magnifications of the boxed areaa in (A,C). Double labeled: red - Pals1; green - Golga2 (assay MGI:5697453).
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Specimen
4G:
postnatal day 10
(more )
Note: Double labeled: red - Pals1; green - Golga2 (assay MGI:5697453).
Specimen
4G:
(close )
Genetic Background:
|
either: C57BL/6J or Swiss Webster
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Age:
|
postnatal day 10
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pals1; green - Golga2 (assay MGI:5697453).
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Specimen
4H:
postnatal day 21
(more )
Note: Double labeled: red - Pals1; green - Golga2 (assay MGI:5697453).
Specimen
4H:
(close )
Genetic Background:
|
either: C57BL/6J or Swiss Webster
|
Age:
|
postnatal day 21
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Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pals1; green - Golga2 (assay MGI:5697453).
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Specimen
6A:
embryonic day 18.5
(more )
Specimen
6A:
(close )
Genetic Background:
|
either: C57BL/6J or Swiss Webster
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: lens epithelium |
Present |
|
6A
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Expression is at the apical surface and at subapical areas in the vicinity of nuclei.
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Specimen
6D:
embryonic day 14.5
(more )
Note: Double labeled: red - Pals1; green - Golga2 (assay MGI:5697453).
Specimen
6D:
(close )
Genetic Background:
|
either: C57BL/6J or Swiss Webster
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pals1; green - Golga2 (assay MGI:5697453).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: lens epithelium |
Present |
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6D
,
6D2
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Expression is at the apical surface and at subapical areas in the vicinity of nuclei.
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