Antibody:

anti-ChAT (AB144P)

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present	Regionally restricted	1f bottom	Expression was detected in starburst amacrine cells.
TS27: retina inner nuclear layer	Present	Regionally restricted	1f bottom	Expression was detected in starburst amacrine cells.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	2a left	Expression was detected in starburst amacrine cells, which were evenly spaced.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	2a middle	Expression was detected in starburst amacrine cells, which exhibited clumps and gaps in their spacing.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	2b top	Expression was detected in the starburst amacrine cells.
TS28: retina inner nuclear layer	Present	Regionally restricted	2b top	Expression was detected in the starburst amacrine cells.
TS28: retina inner plexiform layer	Present	Regionally restricted	2b top	Expression was detected in the starburst amacrine cell dendrites.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	2b bottom	Expression was detected in the starburst amacrine cells that had migrated to the appropriate cell layers as in wild type. There were regions where the somata were clumped in the mutant.
TS28: retina inner nuclear layer	Present	Regionally restricted	2b bottom	Expression was detected in the starburst amacrine cells that had migrated to the appropriate cell layers as in wild type. There were regions where the somata were clumped in the mutant.
TS28: retina inner plexiform layer	Present	Regionally restricted	2b bottom	Expression was detected in the dendrites as in wild type.

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present	Regionally restricted	S1d +/+ right , S1d +/+ left	Expression was detected in starburst amacrine cells.
TS27: retina inner nuclear layer	Present	Regionally restricted	S1d +/+ right , S1d +/+ left	Expression was detected in starburst amacrine cells.

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present	Regionally restricted	S1d -/- left , S1d -/- right	Expression was detected in starburst amacrine cells as in wild type.
TS27: retina inner nuclear layer	Present	Regionally restricted	S1d -/- left , S1d -/- right	Expression was detected in starburst amacrine cells as in wild type.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	S3d left	Expression was detected in the starburst amacrine cells.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	S3d right	Expression was detected in the starburst amacrine cells as in wild type, but there were defects in spacing similar to those in the inner nuclear layer.

Structure	Level	Pattern	Image	Note
TS28: retina inner plexiform layer	Present	Regionally restricted	S3e left	Expression was detected in the starburst amacrine cell processes, which form a meshwork with small holes of relatively constant size. The processes form a dense plexus in two inner plexiform sublaminae.

Structure	Level	Pattern	Image	Note
TS28: retina inner plexiform layer	Present	Regionally restricted	S3e right	Expression was detected in the starburst amacrine cell processes, but the spacing in the mutant plexus shows larger holes of variable sizes and abnormal aggregations corresponding to the defects in the soma array.

Structure	Level	Pattern	Image	Note
TS28: retina layer	Present	Regionally restricted	S4a left	Expression was detected in starburst amacrine cells.

Structure	Level	Pattern	Image	Note
TS28: retina layer	Present	Regionally restricted	S4a right	Expression was detected in starburst amacrine cells. The heterozygous mosaic regularity was mildly disorganized.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	S6a right	Expression was detected in starburst amacrine cells.
TS28: retina inner nuclear layer	Present	Regionally restricted	S6a right	Expression was detected in starburst amacrine cells.
TS28: retina inner plexiform layer	Present	Regionally restricted	S6a right	Expression was detected in starburst amacrine cell dendrites, which project to two sublaminae in the inner plexiform layer.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	S6b right	Expression was detected in starburst amacrine cells.
TS28: retina inner nuclear layer	Present	Regionally restricted	S6b right	Expression was detected in starburst amacrine cells.
TS28: retina inner plexiform layer	Present	Regionally restricted	S6b right	Expression was detected in starburst amacrine cell dendrites, which project to two sublaminae in the inner plexiform layer.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	S6d right	Expression was detected in starburst amacrine cells.
TS28: retina inner nuclear layer	Present	Regionally restricted	S6d right	Expression was detected in starburst amacrine cells.
TS28: retina inner plexiform layer	Present	Regionally restricted	S6d right	Expression was detected in starburst amacrine cell dendrites, which project to two sublaminae in the inner plexiform layer.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	S6e right	Expression was detected in starburst amacrine cells.
TS28: retina inner nuclear layer	Present	Regionally restricted	S6e right	Expression was detected in starburst amacrine cells.
TS28: retina inner plexiform layer	Present	Regionally restricted	S6e right	Expression was detected in starburst amacrine cell dendrites, which project to two sublaminae in the inner plexiform layer.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	S8a	Expression was detected in starburst amacrine cells as in wild type.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	S8b	Expression was detected in starburst amacrine cells. The abnormal mosaic spacing was no different than Megf10-/- mutants.