Chat Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:182578 Kay JN, et al., MEGF10 and MEGF11 mediate homotypic interactions required for mosaic spacing of retinal neurons. Nature. 2012 Mar 22;483(7390):465-9
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5660818
Gene symbol:	Chat
Gene name:	choline O-acetyltransferase

Antibody:

anti-ChAT (AB144P)

Results

Specimen 1f bottom: postnatal day 3 (more

)
Note: Double labeled: red - Chat; green - Megf10 (assay MGI:5660817).

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present	Regionally restricted	1f bottom	Expression was detected in starburst amacrine cells.
TS27: retina inner nuclear layer	Present	Regionally restricted	1f bottom	Expression was detected in starburst amacrine cells.

Specimen 2a wild type: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	2a left	Expression was detected in starburst amacrine cells, which were evenly spaced.

Specimen 2a Megf10-/-: postnatal day 15; Megf10^{tm1a(KOMP)Jrs}/Megf10^{tm1a(KOMP)Jrs} (more

)

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	2a middle	Expression was detected in starburst amacrine cells, which exhibited clumps and gaps in their spacing.

Specimen 2b wild type: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	2b top	Expression was detected in the starburst amacrine cells.
TS28: retina inner nuclear layer	Present	Regionally restricted	2b top	Expression was detected in the starburst amacrine cells.
TS28: retina inner plexiform layer	Present	Regionally restricted	2b top	Expression was detected in the starburst amacrine cell dendrites.

Specimen 2b Megf10-/-: postnatal day 15; Megf10^{tm1a(KOMP)Jrs}/Megf10^{tm1a(KOMP)Jrs} (more

)

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	2b bottom	Expression was detected in the starburst amacrine cells that had migrated to the appropriate cell layers as in wild type. There were regions where the somata were clumped in the mutant.
TS28: retina inner nuclear layer	Present	Regionally restricted	2b bottom	Expression was detected in the starburst amacrine cells that had migrated to the appropriate cell layers as in wild type. There were regions where the somata were clumped in the mutant.
TS28: retina inner plexiform layer	Present	Regionally restricted	2b bottom	Expression was detected in the dendrites as in wild type.

Specimen S1d +/+: postnatal day 3 (more

)
Note: Double labeled: red - Chat; green - Megf10 (assay MGI:5660817).

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present	Regionally restricted	S1d +/+ right , S1d +/+ left	Expression was detected in starburst amacrine cells.
TS27: retina inner nuclear layer	Present	Regionally restricted	S1d +/+ right , S1d +/+ left	Expression was detected in starburst amacrine cells.

Specimen S1d -/-: postnatal day 3; Megf10^{tm1a(KOMP)Jrs}/Megf10^{tm1a(KOMP)Jrs} (more

)
Note: Double labeled: red - Chat; green - Megf10 (assay MGI:5660817).

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present	Regionally restricted	S1d -/- left , S1d -/- right	Expression was detected in starburst amacrine cells as in wild type.
TS27: retina inner nuclear layer	Present	Regionally restricted	S1d -/- left , S1d -/- right	Expression was detected in starburst amacrine cells as in wild type.

Specimen S3d wild type: postnatal day 15-17 (more

)

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	S3d left	Expression was detected in the starburst amacrine cells.

Specimen S3d -/-: postnatal day 15-17; Megf10^{tm1a(KOMP)Jrs}/Megf10^{tm1a(KOMP)Jrs} (more

)

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	S3d right	Expression was detected in the starburst amacrine cells as in wild type, but there were defects in spacing similar to those in the inner nuclear layer.

Specimen S3e wild type: postnatal day 15-17 (more

)

Structure	Level	Pattern	Image	Note
TS28: retina inner plexiform layer	Present	Regionally restricted	S3e left	Expression was detected in the starburst amacrine cell processes, which form a meshwork with small holes of relatively constant size. The processes form a dense plexus in two inner plexiform sublaminae.

Specimen S3e -/-: postnatal day 15-17; Megf10^{tm1a(KOMP)Jrs}/Megf10^{tm1a(KOMP)Jrs} (more

)

Structure	Level	Pattern	Image	Note
TS28: retina inner plexiform layer	Present	Regionally restricted	S3e right	Expression was detected in the starburst amacrine cell processes, but the spacing in the mutant plexus shows larger holes of variable sizes and abnormal aggregations corresponding to the defects in the soma array.

Specimen S4a wild type: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: retina layer	Present	Regionally restricted	S4a left	Expression was detected in starburst amacrine cells.

Specimen S4a +/-: postnatal day 15; Megf10^{tm1a(KOMP)Jrs}/Megf10⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: retina layer	Present	Regionally restricted	S4a right	Expression was detected in starburst amacrine cells. The heterozygous mosaic regularity was mildly disorganized.

Specimen S6a right: postnatal day 14; Megf10^{tm1a(KOMP)Jrs}/Megf10⁺ (more

)
Note: Double labeled: red - Chat; green - Megf10 (assay MGI:5660930).

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	S6a right	Expression was detected in starburst amacrine cells.
TS28: retina inner nuclear layer	Present	Regionally restricted	S6a right	Expression was detected in starburst amacrine cells.
TS28: retina inner plexiform layer	Present	Regionally restricted	S6a right	Expression was detected in starburst amacrine cell dendrites, which project to two sublaminae in the inner plexiform layer.

Specimen S6b right: postnatal day 14; Megf11^{tm1a(EUCOMM)Wtsi}/Megf11⁺ (more

)
Note: Double labeled: red - Chat; green - Megf11 (assay MGI:5660932).

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	S6b right	Expression was detected in starburst amacrine cells.
TS28: retina inner nuclear layer	Present	Regionally restricted	S6b right	Expression was detected in starburst amacrine cells.
TS28: retina inner plexiform layer	Present	Regionally restricted	S6b right	Expression was detected in starburst amacrine cell dendrites, which project to two sublaminae in the inner plexiform layer.

Specimen S6d right: postnatal day 14; Megf10^{tm1a(KOMP)Jrs}/Megf10^{tm1a(KOMP)Jrs} (more

)
Note: Double labeled: red - Chat; green - Megf10 (assay MGI:5660930).

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	S6d right	Expression was detected in starburst amacrine cells.
TS28: retina inner nuclear layer	Present	Regionally restricted	S6d right	Expression was detected in starburst amacrine cells.
TS28: retina inner plexiform layer	Present	Regionally restricted	S6d right	Expression was detected in starburst amacrine cell dendrites, which project to two sublaminae in the inner plexiform layer.

Specimen S6e right: postnatal day 14; Megf11^{tm1a(EUCOMM)Wtsi}/Megf11^{tm1a(EUCOMM)Wtsi} (more

)
Note: Double labeled: red - Chat; green - Megf11 (assay MGI:5660932).

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	S6e right	Expression was detected in starburst amacrine cells.
TS28: retina inner nuclear layer	Present	Regionally restricted	S6e right	Expression was detected in starburst amacrine cells.
TS28: retina inner plexiform layer	Present	Regionally restricted	S6e right	Expression was detected in starburst amacrine cell dendrites, which project to two sublaminae in the inner plexiform layer.

Specimen S8a: postnatal day 15; Megf11^{tm1a(EUCOMM)Wtsi}/Megf11^{tm1a(EUCOMM)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	S8a	Expression was detected in starburst amacrine cells as in wild type.

Specimen S8b: postnatal day 15; Megf10^{tm1a(KOMP)Jrs}/Megf10^{tm1a(KOMP)Jrs}, Megf11^{tm1a(EUCOMM)Wtsi}/Megf11^{tm1a(EUCOMM)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	S8b	Expression was detected in starburst amacrine cells. The abnormal mosaic spacing was no different than Megf10-/- mutants.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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