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Gene Expression Data
Assay Details
Assay
Reference: J:154912 Suzuki H, et al., The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3. Dev Biol. 2009 Dec 15;336(2):222-31
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5645273
Gene symbol: Nanos2
Gene name: nanos C2HC-type zinc finger 2
Antibody: Nanos2 antibody
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1E: postnatal adult (more )
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Clusters 1E Mainly as single cells or two cells connected via intercellular bridges.
Specimen 1F: postnatal adult (more )
Structure Level Pattern Image Note
TS28: seminiferous tubule Weak Clusters 1F Weak expression in chained cells including 4-, 8-, 16-cell clusters.
Specimen 1G: postnatal adult (more )
Structure Level Pattern Image Note
TS28: seminiferous tubule Weak Clusters 1G Weak expression in chained cells including 4-, 8-, 16-cell clusters.
Specimen 2A: postnatal adult (more )
Note: Double labeled: red - Nanos2; green - Gfra1 (assay MGI:5645538).
Structure Level Pattern Image Note
TS28: spermatogonium Present 2A upper , 2A Merge 66.4% coexpressed Gfra1.
Specimen 2B: postnatal adult (more )
Note: Double labeled: red - Nanos2; green - Gfra1 (assay MGI:5645538).
Structure Level Pattern Image Note
TS28: spermatogonium Present 2B upper , 2B Merge 66.4% coexpressed Gfra1.
Specimen S2A: postnatal adult; Nanos2tm2Ysa/Nanos2+ (more )
Note: Double labeled: red - Nanos2 protein; green - Nanos2 reporter (assay MGI:5645139).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present S2A merge , S2A left
Specimen S2B: postnatal adult; Nanos2tm2Ysa/Nanos2+ (more )
Note: Double labeled: red - Nanos2 protein; green - Nanos2 reporter (assay MGI:5645139).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present S2B merge , S2B left
Specimen S6A: postnatal adult (more )
Note: Double labeled: red - Nanos2; green - Gfra1 (assay MGI:5645538).
Structure Level Pattern Image Note
TS28: spermatogonium Present Clusters S6A merge , S6A left Expressed in Apr-like cell clusters.
Specimen S6B: postnatal adult (more )
Note: Double labeled: red - Nanos2; green - Gfra1 (assay MGI:5645538).
Structure Level Pattern Image Note
TS28: spermatogonium Present Clusters S6B left , S6B Merge Expressed in Apr-like cell clusters.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory