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Gene Expression Data
Assay Details
Assay
Reference: J:191315 Courtois A, et al., The transition from meiotic to mitotic spindle assembly is gradual during early mammalian development. J Cell Biol. 2012 Aug 6;198(3):357-70
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5641391
Gene symbol: Pcnt
Gene name: pericentrin (kendrin)
Antibody: Anti-Pericentrin
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: It is possible that a different antibody was used for this assay MGI:3806286.
Results
Specimen 1A: postnatal adult (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS28: secondary oocyte Present Regionally restricted 1A Expression is in microtubule organizing centers.
Specimen 1B: embryonic day 0.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 1B Expression is in microtubule organizing centers.
Specimen 1C: embryonic day 0.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 1C Expression is in microtubule organizing centers.
Specimen 1D: embryonic day 0.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 1D Expression is in microtubule organizing centers.
Specimen 1E: embryonic day 0.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 1E Expression is in microtubule organizing centers.
Specimen 1F: embryonic day 0.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 1F Expression is in microtubule organizing centers.
Specimen 1G: embryonic day 0.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 1G Expression is in microtubule organizing centers.
Specimen 1H: embryonic day 0.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 1H Expression is in microtubule organizing centers.
Specimen 1I: embryonic day 0.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 1I Expression is in microtubule organizing centers.
Specimen 3C left: embryonic day 2.0 (more )
Note: 2-cell to 4-cell. Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS3: 4-cell stage conceptus Present 3C left Expression is in microtubule organizing centers.
Specimen 3C middle: embryonic day 2.0 (more )
Note: 2-cell to 4-cell. Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS3: 4-cell stage conceptus Present 3C middle Expression is in microtubule organizing centers.
Specimen 3C right: embryonic day 2.0 (more )
Note: 2-cell to 4-cell. Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS3: 4-cell stage conceptus Present 3C right Expression is in microtubule organizing centers.
Specimen 3D left: embryonic day 3.0 (more )
Note: 8-cell to 16-cell. Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS4: inner cell mass Present 3D left
Specimen 3D middle: embryonic day 3.0 (more )
Note: 8-cell to 16-cell. Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS4: inner cell mass Present 3D middle Expression is in microtubule organizing centers.
Specimen 3D right: embryonic day 3.0 (more )
Note: 8-cell to 16-cell. Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS4: inner cell mass Present 3D right Expression is in microtubule organizing centers.
Specimen 3E left: embryonic day 4.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS6: embryo Present 3E left Expression is in microtubule organizing centers.
Specimen 3E middle: embryonic day 4.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS6: embryo Present 3E middle Expression is in microtubule organizing centers.
Specimen 3E right: embryonic day 4.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS6: embryo Present 3E right Expression is in microtubule organizing centers.
Specimen 4A 16-cell stage: embryonic day 3.0 (more )
Note: Double labeled: red - Pcnt; green- Cetn1 (assay MGI:5641394). Fixative was 100% methanol.
Structure Level Pattern Image Note
TS4: inner cell mass Present 4A pericentrin 16-cell stage , 4A merge 16-cell stage Expression is in microtubule organizing centers.
Specimen 4A E3.5: embryonic day 3.5 (more )
Note: Double labeled: red - Pcnt; green- Cetn1 (assay MGI:5641394). Fixative was 100% methanol.
Structure Level Pattern Image Note
TS5: inner cell mass Present 4A merge E3.5 , 4A pericentrin E3.5 Expression is in microtubule organizing centers.
Specimen 4A E4.5: embryonic day 4.5 (more )
Note: Double labeled: red - Pcnt; green- Cetn1 (assay MGI:5641394). Fixative was 100% methanol.
Structure Level Pattern Image Note
TS6: embryo Present 4A merge E4.5 , 4A pericentrin E4.5 Expression is in microtubule organizing centers.
Specimen 4B left 16-cell: embryonic day 3.0 (more )
Note: Double labeled: red - Pcnt; green- Ccp110 (assay MGI:5641395). Fixative was 100% methanol.
Structure Level Pattern Image Note
TS4: inner cell mass Present 4B left pericentrin 16-cell stage , 4B left merge 16-cell stage Expression is in microtubule organizing centers.
Specimen 4B left E3.5: embryonic day 3.5 (more )
Note: Double labeled: red - Pcnt; green- Ccp110 (assay MGI:5641395). Fixative was 100% methanol.
Structure Level Pattern Image Note
TS5: inner cell mass Present 4B left pericentrin E3.5 , 4B left merge E3.5 Expression is in microtubule organizing centers.
Specimen 4B left E4.5: embryonic day 4.5 (more )
Note: Double labeled: red - Pcnt; green- Ccp110 (assay MGI:5641395). Fixative was 100% methanol.
Structure Level Pattern Image Note
TS6: embryo Present 4B left pericentrin E4.5 , 4B left merge E4.5 Expression is in microtubule organizing centers.
Specimen 4B right 16-cell: embryonic day 3.0 (more )
Note: Triple labeled: magenta - Pcnt; green - Kif12 (assay MGI:5641396); red - microtubules. Fixative was 100% methanol.
Structure Level Pattern Image Note
TS4: inner cell mass Present 4B right pericentrin 16-cell stage , 4B right merge 16-cell stage Expression is in microtubule organizing centers.
Specimen 4B right E3.5: embryonic day 3.5 (more )
Note: Triple labeled: magenta - Pcnt; green - Kif12 (assay MGI:5641396); red - microtubules.
Structure Level Pattern Image Note
TS5: inner cell mass Present 4B right pericentrin E3.5 , 4B right merge E3.5 Expression is in microtubule organizing centers.
Specimen 4B right E4.5: embryonic day 4.5 (more )
Note: Triple labeled: magenta - Pcnt; green - Kif12 (assay MGI:5641396); red - microtubules.
Structure Level Pattern Image Note
TS6: embryo Present 4B right merge E4.5 , 4B right pericentrin E4.5 Expression is in microtubule organizing centers.
Specimen S3: embryonic day 2.0 (more )
Note: Double labeled: red - Pcnt; green- microtubules.
Structure Level Pattern Image Note
TS3: 8-cell stage conceptus Present S3 Expression is in microtubule organizing centers.
Specimen S4 16-cell stage: embryonic day 3.0 (more )
Note: Double labeled: red - Pcnt; green - Odf2 (assay MGI:5641400).
Structure Level Pattern Image Note
TS4: inner cell mass Present S4_pericentrin 16-cell stage Expression is in microtubule organizing centers.
Specimen S4 E3.5: embryonic day 3.5 (more )
Note: Double labeled: red - Pcnt; green - Odf2 (assay MGI:5641400).
Structure Level Pattern Image Note
TS5: inner cell mass Present S4_pericentrin E3.5 Expression is in microtubule organizing centers.
Specimen S4 E4.5: embryonic day 4.5 (more )
Note: Double labeled: red - Pcnt; green - Odf2 (assay MGI:5641400).
Structure Level Pattern Image Note
TS6: embryo Present S4_pericentrin E4.5 Expression is in microtubule organizing centers.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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