Gene Expression Data
Assay Details
Assay
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Reference:
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J:162095
Shiwaku H, et al., Suppression of the novel ER protein Maxer by mutant ataxin-1 in Bergman glia contributes to non-cell-autonomous toxicity. EMBO J. 2010 Jul 21;29(14):2446-60
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5638224
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Gene symbol:
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Sox9
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Gene name:
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SRY (sex determining region Y)-box 9
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Antibody:
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Anti-Sox9
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
S5A WT 1w:
postnatal week 1
(more )
Note: Double labeled: green - Sox9; red - Calb1 (assay MGI:5638221).
Specimen
S5A WT 1w:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal week 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Sox9; red - Calb1 (assay MGI:5638221).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
S5A WT Sox9 1w
,
S5A WT Merge 1w
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Expression was detected in Bergmann glia.
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Specimen
S5A Atx1-KI 1w:
postnatal week 1; Atxn1tm1Hzo/Atxn1 +
(more )
Note: Double labeled: green - Sox9; red - Calb1 (assay MGI:5638221).
Specimen
S5A Atx1-KI 1w:
(close )
Genetic Background:
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B6.129S7-Atxn1tm1Hzo
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Mutant Allele(s):
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Atxn1tm1Hzo/Atxn1+
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Age:
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postnatal week 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Note:
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Double labeled: green - Sox9; red - Calb1 (assay MGI:5638221).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
S5A Atx1-KI Merge 1w
,
S5A Atx1-KI Sox9 1w
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There was a decrease in Bergmann glia in the mutant.
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Specimen
S5A WT 16w:
postnatal week 16
(more )
Note: Double labeled: green - Sox9; red - Calb1 (assay MGI:5638221).
Specimen
S5A WT 16w:
(close )
Genetic Background:
|
Not Specified
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Age:
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postnatal week 16
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Note:
|
Double labeled: green - Sox9; red - Calb1 (assay MGI:5638221).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
S5A WT Sox9 16w
,
S5A WT Merge 16w
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Expression was detected in Bergmann glia.
|
Specimen
S5A Atx1-KI 16w:
postnatal week 16; Atxn1tm1Hzo/Atxn1 +
(more )
Note: Double labeled: green - Sox9; red - Calb1 (assay MGI:5638221).
Specimen
S5A Atx1-KI 16w:
(close )
Genetic Background:
|
B6.129S7-Atxn1tm1Hzo
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Mutant Allele(s):
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Atxn1tm1Hzo/Atxn1+
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Age:
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postnatal week 16
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Note:
|
Double labeled: green - Sox9; red - Calb1 (assay MGI:5638221).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
S5A Atx1-KI Merge 16w
,
S5A Atx1-KI Sox9 16w
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There was a decrease in Bergmann glia in the mutant.
|
Specimen
S5A WT 40w:
postnatal week 40
(more )
Note: Double labeled: green - Sox9; red - Calb1 (assay MGI:5638221).
Specimen
S5A WT 40w:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal week 40
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Note:
|
Double labeled: green - Sox9; red - Calb1 (assay MGI:5638221).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
S5A WT Sox9 40w
,
S5A WT Merge 40w
|
Expression was detected in Bergmann glia.
|
Specimen
S5A Atx1-KI 40w:
postnatal week 40; Atxn1tm1Hzo/Atxn1 +
(more )
Note: Double labeled: green - Sox9; red - Calb1 (assay MGI:5638221).
Specimen
S5A Atx1-KI 40w:
(close )
Genetic Background:
|
B6.129S7-Atxn1tm1Hzo
|
Mutant Allele(s):
|
Atxn1tm1Hzo/Atxn1+
|
Age:
|
postnatal week 40
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Sox9; red - Calb1 (assay MGI:5638221).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
S5A Atx1-KI Merge 40w
,
S5A Atx1-KI Sox9 40w
|
There was a decrease in Bergmann glia in the mutant.
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