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Gene Expression Data
Assay Details
Assay
Reference: J:162095 Shiwaku H, et al., Suppression of the novel ER protein Maxer by mutant ataxin-1 in Bergman glia contributes to non-cell-autonomous toxicity. EMBO J. 2010 Jul 21;29(14):2446-60
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5638165
Gene symbol: Ufl1
Gene name: UFM1 specific ligase 1
Antibody: C15-01D
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2B upper,middle: embryonic day 17.0 (more )
Note: Double labeled: green - Ufl1; red - Slc1a3 (assay MGI:5638186).
Structure Level Pattern Image Note
TS25: cerebral cortex Present Regionally restricted 2B Merge upper , 2B Maxer middle , 2B Maxer upper , 2B Merge middle There was expression in Glast-positive radial glia.
Specimen 2B lower: embryonic day 17.0 (more )
Note: Double labeled: green - Ufl1; red - Sox2 (assay MGI:5638190).
Structure Level Pattern Image Note
TS25: cerebral cortex Present Regionally restricted 2B Merge lower , 2B Maxer lower Expression was detected in Sox2-positive neural stem cells.
Specimen 2C WT: postnatal week 1 (more )
Note: Double labeled: green - Ufl1; red - Slc1a3 (assay MGI:5638186).
Structure Level Pattern Image Note
TS28: cerebellum Present Regionally restricted 2C WT Maxer , 2C WT Merge Expression was high in Glast-positive Bergmann glia.
Specimen 2C Atx-KI: postnatal week 1; Atxn1tm1Hzo/Atxn1+ (more )
Note: Double labeled: green - Ufl1; red - Slc1a3 (assay MGI:5638186).
Structure Level Pattern Image Note
TS28: cerebellum Present Regionally restricted 2C Atx1-KI Merge , 2C Atx1-KI Maxer Expression was reduced in Bergmann glia in the mutant.
Specimen 2D WT: postnatal week 16 (more )
Note: Double labeled: green - Ufl1; red - Gfap (assay MGI:5638192).
Structure Level Pattern Image Note
TS28: cerebellum Present Regionally restricted 2D WT Maxer , 2D WT Merge Expression was high in Gfap-positive Bergmann glia.
Specimen 2D Atx1-KI: postnatal week 16; Atxn1tm1Hzo/Atxn1+ (more )
Note: Double labeled: green - Ufl1; red - Gfap (assay MGI:5638192).
Structure Level Pattern Image Note
TS28: cerebellum Present Regionally restricted 2D Atx1-KI Maxer , 2D Atx1-KI Merge Expression was reduced in Bergmann glia in the mutant.
Specimen 2F: postnatal week 1 (more )
Note: Double labeled: green - Ufl1; red - Pcna (assay MGI:5638205).
Structure Level Pattern Image Note
TS28: cerebellum Present Regionally restricted 2F Merge , 2F Maxer Expression was detected in PCNA-positive cells.
Specimen S1A: postnatal week 16 (more )
Note: Double labeled: green - Ufl1; red - Calb1 (assay MGI:5638221).
Structure Level Pattern Image Note
TS28: cerebellum Present Regionally restricted S1A Merge , S1A Maxer Expression was in Bergmann glia, but not in Purkinje cells.
TS28: cerebellum Purkinje cell layer Absent S1A Maxer , S1A Merge
Specimen S1B: postnatal week 16 (more )
Note: Double labeled: green - Ufl1; red - Sox2 (assay MGI:5638190).
Structure Level Pattern Image Note
TS28: cerebellum Present Regionally restricted S1B Maxer , S1B Merge Expression was in Sox2-positive Bergmann glia.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory