Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:61910
Kramer PR, et al., Transcription factor activator protein-2 is required for continued luteinizing hormone-releasing hormone expression in the forebrain of developing mice. Endocrinology. 2000 May;141(5):1823-38
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:5636465
|
|
Gene symbol:
|
Gnrh1
|
|
Gene name:
|
gonadotropin releasing hormone 1
|
|
Probe:
|
LHRH probe
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
|
Results
|
Specimen
5A:
embryonic day 13.5
(more  )
Specimen
5A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
5B:
(close )
|
Genetic Background:
|
either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
|
|
Mutant Allele(s):
|
Tfap2atm1Jae/Tfap2atm1Jae
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forebrain |
Present |
Single cells |
5B
|
Expression is as is wild type.
|
Specimen
5C:
embryonic day 14.5
(more )
Specimen
5C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
5D:
(close )
|
Genetic Background:
|
either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
|
|
Mutant Allele(s):
|
Tfap2atm1Jae/Tfap2atm1Jae
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forebrain |
Present |
Single cells |
5D
|
The number of expressing cells is significantly decreased in the mutant.
|
Specimen
not shown:
embryonic day 13.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: nose |
Present |
|
|
Expression is in nasal regions.
|
Specimen
not shown:
(close )
|
Genetic Background:
|
either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
|
|
Mutant Allele(s):
|
Tfap2atm1Jae/Tfap2atm1Jae
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: nose |
Present |
|
|
Expression is the same as in wild type; it is present in nasal regions.
|
Specimen
not shown:
embryonic day 14.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: nose |
Present |
|
|
Expression is in nasal regions.
|
Specimen
not shown:
(close )
|
Genetic Background:
|
either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
|
|
Mutant Allele(s):
|
Tfap2atm1Jae/Tfap2atm1Jae
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: nose |
Present |
|
|
Expression is the same as in wild type; it is present in nasal regions.
|
Specimen
7A:
embryonic day 14.5
(more )
Note: Overlay and colorization of adjacent sections; red - Gnrh1; blue - Gal (assay MGI:5636470).
Specimen
7A:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Overlay and colorization of adjacent sections; red - Gnrh1; blue - Gal (assay MGI:5636470).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forebrain |
Present |
Single cells |
7A
|
|
| TS22: nose |
Present |
Single cells |
7A
|
Expression is present in the nasal region.
|
Specimen
7B:
(close )
|
Genetic Background:
|
either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
|
|
Mutant Allele(s):
|
Tfap2atm1Jae/Tfap2atm1Jae
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Overlay and colorization of adjacent sections; red - Gnrh1; blue - Gal (assay MGI:5636470).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forebrain |
Present |
Single cells |
7B
|
|
| TS22: nose |
Present |
Single cells |
7B
|
Expression is present in the nasal region.
|
Specimen
7E:
embryonic day 13.5
(more )
Note: Overlay and colorization of adjacent sections; red - Gnrh1; blue - Gal (assay MGI:5636470).
Specimen
7E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Overlay and colorization of adjacent sections; red - Gnrh1; blue - Gal (assay MGI:5636470).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forebrain |
Present |
|
7E
|
Areas of expression include nasal tracts.
|
| TS21: nose |
Present |
Regionally restricted |
7E
|
Expression is in the olfactory pit.
|
Specimen
7F:
embryonic day 13.5
(more )
Note: Overlay and colorization of adjacent sections; red - Gnrh1; blue - Gal (assay MGI:5636470).
Specimen
7F:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Overlay and colorization of adjacent sections; red - Gnrh1; blue - Gal (assay MGI:5636470).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forebrain |
Present |
|
7F
|
|
| TS21: nose |
Present |
Regionally restricted |
7F
|
Expression is in the olfactory pit.
|
|
Analysis Tools