Gene Expression Data
Assay Details
Assay
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Reference:
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J:61910
Kramer PR, et al., Transcription factor activator protein-2 is required for continued luteinizing hormone-releasing hormone expression in the forebrain of developing mice. Endocrinology. 2000 May;141(5):1823-38
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5636459
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Gene symbol:
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Gnrh1
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Gene name:
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gonadotropin releasing hormone 1
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Antibody:
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anti-pro-LHRH antibody (SW-1)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Assay notes:
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The authors described two antibodies but did not specify which was used to generate these data; it is possible MGI:5467573 was used instead.
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Results
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Specimen
3A/C:
embryonic day 13.5
(more )
Note: Double labeled: red - Gnrh1; green - Tfap2a (assay MGI:5636458).
Specimen
3A/C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
|
Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: red - Gnrh1; green - Tfap2a (assay MGI:5636458).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: nasal cavity |
Present |
Single cells |
3A
,
3C
|
Expression present at the nasal/forebrain junction (at the cribriform plate).
|
Specimen
3E/G:
embryonic day 15.5
(more )
Note: Double labeled: red - Gnrh1; green - Tfap2a (assay MGI:5636458).
Specimen
3E/G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gnrh1; green - Tfap2a (assay MGI:5636458).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: nasal cavity |
Present |
Single cells |
3E
,
3G
|
Expression present at the nasal/forebrain junction (at the cribriform plate).
|
Specimen
4A/C:
embryonic day 14.5
(more )
Note: Double labeled: red - Gnrh1; green - Tfap2a (assay MGI:5636458).
Specimen
4A/C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gnrh1; green - Tfap2a (assay MGI:5636458).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: forebrain |
Present |
Single cells |
4A
,
4C
|
|
Specimen
4D/F:
embryonic day 14.5
(more )
Note: Double labeled: red - Gnrh1; green - Tfap2a (assay MGI:5636458).
Specimen
4D/F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gnrh1; green - Tfap2a (assay MGI:5636458).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: forebrain |
Present |
Single cells |
4D
,
4F
|
|
Specimen
4H/J:
postnatal day 1
(more )
Note: Double labeled: red - Gnrh1; green - Tfap2a (assay MGI:5636458).
Specimen
4H/J:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gnrh1; green - Tfap2a (assay MGI:5636458).
|
|