Antibody:	anti-Dab2
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS22: subcommissural organ	Present		2M , 2M''	There was no co-localization with PGP9.5.
TS22: posterior commissure	Absent		2M'' , 2M

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Strong	Scattered	3A , 3A''	Scattered strongly labeled cells were detected in the neuroepithelium that co-expressed Iba1 (a mononuclear phagocyte marker).
TS17: embryo mesenchyme	Present		3A'' , 3A	Expression was in Iba1-positive fetal macrophages outside the neural tube.

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present		3B	Immunoreactive cells did not express PGP9.5, indicating they were not early differentiating neurons.

Structure	Level	Pattern	Image	Note
TS20: embryo mesenchyme	Present		3C	Expression was found in Iba1-positive fetal macrophages.

Structure	Level	Pattern	Image	Note
TS22: embryo mesenchyme	Present		3H , 3H''	Expression was in Iba1+ macrophages in the mesenchyme.
TS22: central nervous system	Absent		3H'' , 3H	Expression was absent from microglial progenitors within the neural tube.

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	3L'' , 3L	Expression co-localized with some, but not all, F4/80-positive mature macrophages on the wall of cerebral microvessels.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present		3N'' , 3N	Expression co-localized with Iba1-positive macrophages.

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Present		3O , 3O''	Expression co-localized with F4/80-positive macrophages.