Gene Expression Data
Assay Details
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Assay
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Reference:
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J:30430
Schonemann MD, et al., Development and survival of the endocrine hypothalamus and posterior pituitary gland requires the neuronal POU domain factor Brn-2. Genes Dev. 1995 Dec 15;9(24):3122-35
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5620304
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Gene symbol:
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Pou3f4
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Gene name:
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POU domain, class 3, transcription factor 4
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Antibody:
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4R3 rabbit anti-Brn4
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
2A upper middle:
embryonic day 16.0
(more  )
Specimen
2A upper middle:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hypothalamus |
Present |
Regionally restricted |
2A upper middle
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Expressed in neurons of the nascent paraventricular nucleus. Also expressed in neurons immediately ventral to the paraventricular neurons.
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Specimen
2A lower middle:
postnatal day 5
(more )
Specimen
2A lower middle:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
6B upper left:
embryonic day 13.0
(more )
Specimen
6B upper left:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hypothalamus |
Present |
Regionally restricted |
6B upper left
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Expressed in the paraventricular hypothalamus neurons.
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Specimen
6B lower left:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Pou3f2tm1Rsd/Pou3f2tm1Rsd
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hypothalamus |
Present |
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6B lower left
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Equivalently expressed in the paraventricular hypothalamus neurons, compared with the wild-type.
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Specimen
6B upper middle:
embryonic day 17.0
(more )
Specimen
6B upper middle:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus |
Present |
Regionally restricted |
6B upper middle
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Expressed in the paraventricular hypothalamus neurons.
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Specimen
6B lower middle:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Pou3f2tm1Rsd/Pou3f2tm1Rsd
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Age:
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embryonic day 17.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus |
Present |
Scattered |
6B lower middle
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Mostly absent in the presumptive paraventricular hypothalamus neurons. Only expressed in a few scattered cells.
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Specimen
6B upper right:
postnatal day 5
(more )
Specimen
6B upper right:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
6B lower right:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Pou3f2tm1Rsd/Pou3f2tm1Rsd
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: paraventricular hypothalamic nucleus |
Trace |
Scattered |
6B lower right
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Mostly absent in the presumptive paraventricular hypothalamus neurons. Only expressed in a few scattered cells.
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Specimen
not shown:
postnatal
(more )
Note: Also stained for Trh (assay MGI:5620402).
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Also stained for Trh (assay MGI:5620402).
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Analysis Tools