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Gene Expression Data
Assay Details
Assay
Reference: J:30430 Schonemann MD, et al., Development and survival of the endocrine hypothalamus and posterior pituitary gland requires the neuronal POU domain factor Brn-2. Genes Dev. 1995 Dec 15;9(24):3122-35
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5620268
Gene symbol: Pou3f2
Gene name: POU domain, class 3, transcription factor 2
Antibody: anti-Brn-2
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Results
Specimen 1c: embryonic day 16.0 (more )
Structure Level Pattern Image Note
TS24: cerebral cortex subventricular zone Strong 1c
TS24: cortical plate Strong 1c
Specimen 1d: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: cortical layer II Present 1d
TS28: cortical layer III Present 1d
TS28: cortical layer V Present 1d
Specimen 1e: postnatal (more )
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Strong 1e
Specimen 1f: postnatal (more )
Structure Level Pattern Image Note
TS28: supraoptic nucleus Strong 1f
Specimen 1g/g': postnatal day 5 (more )
Note: Double labeled: brown - Pou3f2; blue - Crh (assay MGI:5620363).
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present 1g , 1g' Near complete coexpression with Crh.
Specimen 1h/h': postnatal day 5 (more )
Note: Double labeled: brown - Pou3f2; blue - Avp (assay MGI:5620365).
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present 1h , 1h' Near complete coexpression with Avp.
Specimen 1i/i': postnatal day 5 (more )
Note: Double labeled: brown - Pou3f2; blue - Oxt (assay MGI:5620366).
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present 1i , 1i' Near complete coexpression with Oxt.
Specimen 2A upper left: embryonic day 16.0 (more )
Structure Level Pattern Image Note
TS24: hypothalamus Present Regionally restricted 2A upper left Expressed in neurons of the nascent paraventricular nucleus.
Specimen 2A lower left: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present 2A lower left
Specimen 2C left: postnatal (more )
Note: Double labeled: brown - Pou3f2; black - Avp (assay MGI:5620373).
Structure Level Pattern Image Note
TS28: supraoptic nucleus Present 2C left
Specimen 2C middle: postnatal (more )
Note: Double labeled: brown - Pou3f2; black - Oxt (assay MGI:5620374).
Structure Level Pattern Image Note
TS28: supraoptic nucleus Present 2C middle
Specimen 3D upper: postnatal (more )
Structure Level Pattern Image Note
TS28: cerebral cortex Present 3D upper
Specimen 3D lower: postnatal; Pou3f2tm1Rsd/Pou3f2tm1Rsd (more )
Structure Level Pattern Image Note
TS28: cerebral cortex Absent 3D lower
Specimen 3E upper left: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: cortical layer II Present 3E upper left
TS28: cortical layer III Present 3E upper left
TS28: cortical layer V Present 3E upper left
Specimen 3E lower left: postnatal day 5; Pou3f2tm1Rsd/Pou3f2tm1Rsd (more )
Structure Level Pattern Image Note
TS28: cortical layer II Absent 3E lower left
TS28: cortical layer III Absent 3E lower left
TS28: cortical layer V Absent 3E lower left
Specimen not shown: postnatal (more )
Note: Also stained for Trh (assay MGI:5620402).
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present Not coexpressed in a significant number with Trh.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory