About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:196070 Tochitani S, et al., Immunoreactivity for GABA, GAD65, GAD67 and Bestrophin-1 in the meninges and the choroid plexus: implications for non-neuronal sources for GABA in the developing mouse brain. PLoS One. 2013;8(2):e56901
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5614462
Gene symbol: Gad1
Gene name: glutamate decarboxylase 1
Antibody: Anti-GAD65/67 C20 sc-7513
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: The antibody recognizes both Gad1 and Gad2.
Results
Specimen 2A: embryonic day 14.5 (more )
Note: Double labeled: green - Gad1 and Gad2; red - laminin.
Structure Level Pattern Image Note
TS22: telencephalon meninges Present Regionally restricted 2A Expression was along the basal lamina (laminin-positive, red).
Specimen 2B: embryonic day 14.5 (more )
Note: Double labeled: green - Gad1 and Gad2; red - Pan-Zic.
Structure Level Pattern Image Note
TS22: telencephalon meninges Present 2B Most of the labeled cells co-express Zic proteins (red - meningeal fibroblasts), but some immunolabeled cells do not express Zic proteins.
Specimen 2D: embryonic day 14.5 (more )
Note: Double labeled: green - Gad1 and Gad2; red - Rdh10 (assay MGI:5614465).
Structure Level Pattern Image Note
TS22: telencephalon meninges Present 2D Merged , 2D GAD65/67
Specimen 5A: embryonic day 14.5 (more )
Note: Double labeled: green - Gad1 and Gad2; red - Best1 (assay MGI:5614495).
Structure Level Pattern Image Note
TS22: telencephalon meninges Present 5A Merged , 5A Gad65/67
Specimen 5B: embryonic day 14.5 (more )
Note: Double labeled: green - Gad1 and Gad2; red - Best1 (assay MGI:5614495).
Structure Level Pattern Image Note
TS22: choroid plexus Ambiguous 5B Merged , 5B Gad65/67 Other data in this publication (Fig. 4C) indicates that few signals for Gad1 were detected in choroid plexus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory