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Gene Expression Data
Assay Details
Assay
Reference: J:191140 Grigoryan M, et al., Phenotype of entero-endocrine L cells becomes restricted during development. Dev Dyn. 2012 Dec;241(12):1986-92
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5588721
Gene symbol: Gcg
Gene name: glucagon
Antibody: anti-GLP-1
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1A: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: intestine epithelium Present Single cells 1A Expressed in occasional cells (one possitive cell every few sections of intestine).
Specimen 1B: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: intestine epithelium Present 1B More abundant expression in both crypt and villus(2.8 +/- 0.4 possitive cells/100 epithelial cells).
Specimen 1D/E: embryonic day 16.5 (more )
Note: Double labeled: red - GLP-1 (Gcg); green - Gip (assay MGI:5588829).
Structure Level Pattern Image Note
TS24: intestine mucosa Present 1D , 1E Coexpressed Gip.
TS24: ileum Present Regionally restricted 1D , 1E Coexpressed Gip in the mucosa.
Specimen 2C: embryonic day 16.5 (more )
Note: Double labeled: red - GLP-1 (Gcg); green - Pax6 (assay MGI:5602956).
Structure Level Pattern Image Note
TS24: ileum Present 2C Coexpressed Pax6 (81%, see Table 1).
Specimen 2D: embryonic day 16.5 (more )
Note: Double labeled: red - GLP-1 (Gcg); green - Pdx1 (assay MGI:5602877).
Structure Level Pattern Image Note
TS24: ileum Present 2D Coexpressed Pdx1 (44.8%, see Table 1).
Specimen 2E: embryonic day 16.5 (more )
Note: Double labeled: red - GLP-1 (Gcg); green - Mki67 (assay MGI:5602957).
Structure Level Pattern Image Note
TS24: ileum Present 2E No coexpression with Mki67 (large majority).
Specimen 2F: embryonic day 16.5 (more )
Note: Double labeled: red - GLP-1 (Gcg); green - Mki67 (assay MGI:5602957).
Structure Level Pattern Image Note
TS24: ileum Present 2F No coexpression with Mki67 (large majority of L cells).
Specimen 2G/H: embryonic day 16.5 (more )
Note: Double labeled: red - GLP-1 (Gcg); green - Mki67 (assay MGI:5602957).
Structure Level Pattern Image Note
TS24: ileum Present 2G , 2H Coexpressed Mki67 (4% of L cells).
Specimen 4H/J: postnatal month 3 (more )
Note: Double labeled: red - GLP-1 (Gcg); green - Pcsk2 (assay MGI:5602964).
Structure Level Pattern Image Note
TS28: ileum Present 4J , 4H No coexpression with Pcsk2.
Specimen not shown: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: intestine epithelium Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory