Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:176205
Moniot B, et al., Hematopoietic prostaglandin D synthase (H-Pgds) is expressed in the early embryonic gonad and participates to the initial nuclear translocation of the SOX9 protein. Dev Dyn. 2011 Oct;240(10):2335-43
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:5582925
|
Gene symbol:
|
Sox9
|
Gene name:
|
SRY (sex determining region Y)-box 9
|
Antibody:
|
anti-Sox9
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
Results
|
Specimen
2A:
embryonic day 11.5
(more )
Note: 16-17 tail somites. Double labeled: red - Sox9; green - Hpgds (assay MGI:5582812).
Specimen
2A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 11.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
16-17 tail somites. Double labeled: red - Sox9; green - Hpgds (assay MGI:5582812).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: gonad |
Present |
|
2A
|
Expressed in the Sertoli cells.
|
Specimen
2C:
embryonic day 13.5
(more )
Note: Double labeled: red - Sox9; green - Hpgds (assay MGI:5582812).
Specimen
2C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Sox9; green - Hpgds (assay MGI:5582812).
|
Specimen
2E:
embryonic day 17.5
(more )
Note: Double labeled: red - Sox9; green - Hpgds (assay MGI:5582812).
Specimen
2E:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 17.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Sox9; green - Hpgds (assay MGI:5582812).
|
Specimen
3A E11.5 XY left:
embryonic day 11.5
(more )
Note: 17 tail somites. Triple labeled: red - Sox9; green - Hpgds (assay MGI:5583026); blue - Pou5f1 (assay MGI:5583878).
Specimen
3A E11.5 XY left:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 11.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
17 tail somites. Triple labeled: red - Sox9; green - Hpgds (assay MGI:5583026); blue - Pou5f1 (assay MGI:5583878).
|
Specimen
3B left:
embryonic day 11.5
(more )
Note: 17 tail somites. Double labeled: red - Sox9; green - Hpgds (assay MGI:5583026).
Specimen
3B left:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 11.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
17 tail somites. Double labeled: red - Sox9; green - Hpgds (assay MGI:5583026).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: gonad |
Present |
Regionally restricted |
3B left
|
Expressed in the Sertoli cells. Expressed only at the center of the mid E11.5 XY gonad.
|
Specimen
4A upper left:
embryonic day 11.5
(more )
Note: 18 tail somites.
Specimen
4A upper left:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 11.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
18 tail somites.
|
Specimen
4A upper right:
embryonic day 11.5
(more )
Note: 18 tail somites.
Specimen
4A upper right:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 11.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
18 tail somites.
|
Specimen
4B upper left:
embryonic day 12.5
(more )
Specimen
4B upper left:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
4B upper right:
embryonic day 12.5
(more )
Specimen
4B upper right:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5A upper:
embryonic day 11.5
(more )
Specimen
5A upper:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 11.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: gonad |
Present |
|
5A upper
|
Expressed in the nuclei of the Sertoli cells.
|
Specimen
5A lower:
(close )
Genetic Background:
|
B6.129-Hpgdstm1Urad
|
Mutant Allele(s):
|
Hpgdstm1Urad/Hpgdstm1Urad
|
Age:
|
embryonic day 11.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: gonad |
Present |
|
5A lower
|
Expressed in the cytoplasmic compartment in most of the Sertoli cells, whereas a few cells in the nuclei.
|
Specimen
5B left:
embryonic day 12.5
(more )
Specimen
5B left:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5B right:
(close )
Genetic Background:
|
B6.129-Hpgdstm1Urad
|
Mutant Allele(s):
|
Hpgdstm1Urad/Hpgdstm1Urad
|
Age:
|
embryonic day 12.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: Sertoli cell |
Sertoli cell |
Present |
|
5B right
|
Nuclear localization was fully restored.
|
|