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Gene Expression Data
Assay Details
Assay
Reference: J:136395 Zordan P, et al., Comparative analysis of proneural gene expression in the embryonic cerebellum. Dev Dyn. 2008 Jun;237(6):1726-35
Assay type: RNA in situ
MGI Accession ID: MGI:5560710
Gene symbol: Ascl1
Gene name: achaete-scute family bHLH transcription factor 1
Probe: Mash1 cDNA11
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 1B: embryonic day 10.75 (more )
Structure Level Pattern Image Note
TS18: cerebellum primordium Present Regionally restricted 1B Expression was detected in the ventricular zone and in the prospective cerebellar nuclei.

Specimen 2D: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: cerebellum primordium Present Regionally restricted 2D Expression was detected in the ventricular zone.

Specimen 3A: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: cerebellum primordium Present Regionally restricted 3A Expression was detected in the ventricular zone.

Specimen 3A': embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: cerebellum primordium Present Regionally restricted 3A' Expression was detected in the ventricular zone.

Specimen not shown: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: cerebellum primordium Present Regionally restricted Expression was detected in the ventricular zone but was excluded from the rhombic lip.

Specimen 4C, B/C inlay: embryonic day 12.5 (more )
Note: Inlay - Adjacent sections were pseudocolored and superimposed. Colors: green - Ascl1; red - Neurog1 (assay MGI:5561118).
Structure Level Pattern Image Note
TS20: cerebellum primordium Present Regionally restricted 4C , 4B/C overlay Expression was detected in the ventricular zone in a lateral section. There was overlap with Ngn1.

Specimen 4C', B'/C'inlay: embryonic day 12.5 (more )
Note: Inlay - Adjacent sections were pseudocolored and superimposed. Colors: green - Ascl1; red - Neurog1 (assay MGI:5561118).
Structure Level Pattern Image Note
TS20: cerebellum primordium Present Regionally restricted 4B'/C' overlay , 4C' Expression was detected in the ventricular zone in a medial section. There was overlap with Ngn1.

Specimen 4H, G/H inlay: embryonic day 12.5 (more )
Note: Inlay - Adjacent sections were pseudocolored and superimposed. Colors: green - Ascl1; red - Neurog2 (assay MGI:5560711).
Structure Level Pattern Image Note
TS20: cerebellum primordium Present Regionally restricted 4G/H overlay , 4H Expression was detected in the ventricular zone in a lateral section. There was overlap with Ngn2.

Specimen 4H', G'/H' inlay: embryonic day 12.5 (more )
Note: Inlay - Adjacent sections were pseudocolored and superimposed. Colors: green - Ascl1; red - Neurog2 (assay MGI:5560711).
Structure Level Pattern Image Note
TS20: cerebellum primordium Present Regionally restricted 4H' , 4G'/H' overlay Expression was detected in the ventricular zone in a medial section. There was overlap with Ngn2.

Specimen 8D, 8C inlay: embryonic day 13.5 (more )
Note: Fig. 8C Inlay - Adjacent sections were pseudocolored and superimposed. Colors: green - Ascl1; red - Neurog1 (assay MGI:5561118); blue - Pax2 (assay MGI:5561119).
Structure Level Pattern Image Note
TS21: cerebellum ventricular layer Weak 8D , 8C inlay Expression was detected throughout the ventricular zone but was considerably downregulated.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/21/2024
MGI 6.23
The Jackson Laboratory