Probe:	Sfrp2 cDNA5
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Present	Non-Uniform	5a	Expression was strong in the proximal core and then extended distally. Expression was tightly associated with the prechondrogenic cell condensations.

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Present	Non-Uniform	5c	Expression was strong in the proximal core and then extended distally. Expression was tightly associated with the prechondrogenic cell condensations. Expression was mutually exclusive with Sox9 in the perichondrium.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	S1a FL	Expression was tightly associated with the prechondrogenic cell condensations.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	S1a HL	Expression was tightly associated with the prechondrogenic cell condensations.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	S1b FL	Expression was tightly associated with the prechondrogenic cell condensations.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	S1b HL	Expression was tightly associated with the prechondrogenic cell condensations.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted	S1c FL	Expression was tightly associated with the prechondrogenic cell condensations.

Structure	Level	Pattern	Image	Note
TS20: hindlimb mesenchyme	Present	Regionally restricted	S1c HL	Expression was tightly associated with the prechondrogenic cell condensations.

Structure	Level	Pattern	Image	Note
TS21: forelimb mesenchyme	Present	Regionally restricted	S1d FL	Expression was tightly associated with the prechondrogenic cell condensations.

Structure	Level	Pattern	Image	Note
TS21: hindlimb mesenchyme	Present	Regionally restricted	S1d HL	Expression was tightly associated with the prechondrogenic cell condensations.

Structure	Level	Pattern	Image	Note
TS22: forelimb mesenchyme	Present	Regionally restricted	S1e FL	Expression was tightly associated with the prechondrogenic cell condensations.

Structure	Level	Pattern	Image	Note
TS22: hindlimb mesenchyme	Present	Regionally restricted	S1e HL	Expression was tightly associated with the prechondrogenic cell condensations.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted	S2a FL	Expression appeared proximal and distal to each prechondrogenic condensation in a proximo-distal orientation.

Structure	Level	Pattern	Image	Note
TS20: hindlimb mesenchyme	Present	Regionally restricted	S2a HL	Expression appeared proximal and distal to each prechondrogenic condensation in a proximo-distal orientation.

Structure	Level	Pattern	Image	Note
TS21: hand joint	Present	Regionally restricted	S2b FL	Expression was detected in the joint interzone area.

Structure	Level	Pattern	Image	Note
TS21: foot joint	Present	Regionally restricted	S2b HL	Expression was detected in the joint interzone area.

Structure	Level	Pattern	Image	Note
TS22: hand joint	Present	Regionally restricted	S2c FL	Expression was detected in the joint interzone area.

Structure	Level	Pattern	Image	Note
TS22: foot joint	Present	Regionally restricted	S2c HL	Expression was detected in the joint interzone area.

Structure	Level	Pattern	Image	Note
TS23: hand joint	Present	Regionally restricted	S3c FL	Expression was detected in the presumptive joint area.
TS23: forelimb digit	Present	Regionally restricted	S3c FL	Expression was detected in the perichondrocytic area in the digital rays.

Structure	Level	Pattern	Image	Note
TS23: foot joint	Present	Regionally restricted	S3c HL	Expression was detected in the presumptive joint area.
TS23: hindlimb digit	Present	Regionally restricted	S3c HL	Expression was detected in the perichondrocytic area in the digital rays.