Antibody:	anti-FGFR-1
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	Confocal micrographs. Authors state that the antibody cross-reacts with 2 other FGF receptors but did not provide additional details.

Structure	Level	Pattern	Image	Note
TS21: retina	Present	Regionally restricted	1A	Expression is in the retinal axon layer, optic disc, and in peripheral regions of the retina.
TS21: lens	Present		1A

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	1B	Expression is in the retinal axon layer and in peripheral regions of the retina.
TS22: lens	Present		1B
TS22: optic disc	Present		1B

Structure	Level	Pattern	Image	Note
TS23: retina	Present	Regionally restricted	1C	Expression is in the retinal axon layer, ganglion cell layer, and in peripheral regions of the retina.
TS23: lens	Present		1C
TS23: optic disc	Present		1C

Structure	Level	Pattern	Image	Note
TS24: retina	Present	Regionally restricted	1F , 1E	There is a laminated pattern of expression in the inner regions of the retina.
TS24: neural retina	Present	Regionally restricted	1E , 1F	Expression is in the inner plexiform layer.
TS24: retina nerve fiber layer	Present		1F , 1E

Structure	Level	Pattern	Image	Note
TS27: retina	Present	Regionally restricted	1G , 1H	There is a laminated pattern of expression in the inner regions of the retina.
TS27: optic disc	Very strong		1G , 1H
TS27: retina inner plexiform layer	Very strong		1G , 1H

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Weak	Regionally restricted	2A , 2B	Expression is as an inverted V-shape in the posterior region of the diencephalon and at the junction of the stalk and the chiasm.
TS21: optic stalk	Weak		2B

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	2D , 2C	Expression is in the posterior region of the diencephalon.

Structure	Level	Pattern	Image	Note
TS21: optic chiasma	Present		2E

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Very strong	Regionally restricted	3A , 3B	Expression is in the optic tract and the lateral regions of the diencephalon caudal to the tract.
TS22: optic tract	Very strong	Regionally restricted	3A , 3B	Expression is in the optic tract and the lateral regions of the diencephalon caudal to the tract.
TS22: optic chiasma	Weak		3A
TS22: optic stalk	Weak		3A

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Weak	Regionally restricted	3C	Expression is at the optic axon layer and at the midline in this more anterior section.
TS22: optic tract	Weak	Regionally restricted	3C	Expression is at the optic axon layer and at the midline in this more anterior section.

Structure	Level	Pattern	Image	Note
TS22: optic chiasma	Strong	Regionally restricted	3D	In this more caudal section than 3C expression is at the lateral regions of the chiasm close to the threshold of the optic tract.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Weak	Regionally restricted	3E

Structure	Level	Pattern	Image	Note
TS22: optic tract	Present		4E , 4C , 4A
TS22: diencephalon	Present	Regionally restricted	4A , 4C , 4E	Expression was detected in the lateral part of the diencephalon.

Structure	Level	Pattern	Image	Note
TS23: optic tract	Strong		5A , 5B
TS23: diencephalon	Present	Regionally restricted	5B , 5A	Expression is in the diencephalon adjacent to the optic tract.

Structure	Level	Pattern	Image	Note
TS23: optic chiasma	Present		5C
TS23: optic tract	Present		5C
TS23: optic stalk	Weak		5C
TS23: diencephalon	Present	Regionally restricted	5C	There is an increase in staining at the deep region of the optic fiber layer at the midchiasm.

Structure	Level	Pattern	Image	Note
TS23: optic chiasma	Present		5D
TS23: optic tract	Present		5D
TS23: optic stalk	Weak		5D
TS23: diencephalon	Present	Regionally restricted	5D	There is an increase in staining at the deep region of the optic fiber layer at the midchiasm.

Structure	Level	Pattern	Image	Note
TS23: optic chiasma	Present		5E
TS23: optic tract	Present		5E
TS23: optic stalk	Weak		5E
TS23: diencephalon	Weak	Regionally restricted	5E	There is an increase in staining at the deep region of the optic fiber layer at the midchiasm.

Structure	Level	Pattern	Image	Note
TS23: optic tract	Present		5F

Structure	Level	Pattern	Image	Note
TS24: optic stalk	Weak		6A , 6B
TS24: optic tract	Present		6A , 6B

Structure	Level	Pattern	Image	Note
TS24: optic stalk	Weak		6C
TS24: optic tract	Present		6C

Structure	Level	Pattern	Image	Note
TS24: optic stalk	Weak		6D
TS24: optic tract	Present		6D