About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:187373 Plachez C, et al., Expression of nuclear factor one A and -B in the olfactory bulb. J Comp Neurol. 2012 Oct 1;520(14):3135-49
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5510846
Gene symbol: Nfia
Gene name: nuclear factor I/A
Antibody: Anti-NFIA
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 3C/D: postnatal adult; Gad1tm1.1Tama/Gad1+ (more )
Note: Triple labeled: magenta - Nfia; green - Gad1 reporter (assay MGI:5510847); blue - DAPI.
Structure Level Pattern Image Note
TS28: main olfactory bulb Present 3D , 3C Expression in the olfactory bulb did not coincide with GAD67-GFP expression.

Specimen 3G/H: postnatal adult (more )
Note: Triple labeled: magenta - Nfia; green - Tubb3 (assay MGI:5510853); blue - DAPI.
Structure Level Pattern Image Note
TS28: main olfactory bulb Present Regionally restricted 3H , 3G Expression did not coincide with TuJ1-positive mitral cells in the olfactory bulb.

Specimen 3K/L: postnatal adult (more )
Note: Triple labeled: magenta - Nfia; green - Gfap (assay MGI:5510854); blue - DAPI.
Structure Level Pattern Image Note
TS28: main olfactory bulb internal plexiform layer Present Regionally restricted 3L , 3K Expression was detected in nuclei of cells surrounded by GFAP-expressing astrocytic fibers in the internal plexiform layer.
TS28: main olfactory bulb external plexiform layer Present Scattered 3L , 3K Some immunopositive cells were detected in the mitral cell layer and the external plexiform layer that were not GFAP-expressing astrocytes.
TS28: main olfactory bulb mitral cell layer Present Scattered 3L , 3K Some immunopositive cells were detected in the mitral cell layer and the external plexiform layer that were not GFAP-expressing astrocytes.

Specimen 7B: postnatal adult (more )
Note: Double labeled: magenta - Nfia; green - Gfap (assay MGI:5510854).
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Present Regionally restricted 7B Expression was detected in cells lining the lateral ventricles. Some immunopositive cells were surrounded by GFAP-positive fibers, indicating that these may be neural progenitor cells.

Specimen 7C: postnatal adult (more )
Note: Double labeled: magenta - Nfia; green - Gfap (assay MGI:5510854).
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Present Regionally restricted 7C Expression was detected in cells lining the lateral ventricles. Some immunopositive cells were surrounded by GFAP-positive fibers, indicating that these may be neural progenitor cells.

Specimen 8C/D: postnatal adult; Gad1tm1.1Tama/Gad1+ (more )
Note: Triple labeled: magenta - Nfia; green - Gad1 reporter (assay MGI:5510847); blue - DAPI.
Structure Level Pattern Image Note
TS28: rostral migratory stream Present Regionally restricted 8C , 8D Expression was detected in cells of the rostral migratory stream. Some cells were also positive for GAD67-GFP, however some cells were GFP-negative.

Specimen 8K/L: postnatal adult (more )
Note: Triple labeled: magenta - Nfia; green - Gfap (assay MGI:5510854); blue - DAPI.
Structure Level Pattern Image Note
TS28: rostral migratory stream Present Regionally restricted 8L , 8K Expression was detected in cells of the rostral migratory stream. Some immunopositive cells were also GFAP-expressing astrocytes.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
05/14/2024
MGI 6.23
The Jackson Laboratory