Gene Expression Data
Assay Details
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Assay
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Reference:
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J:82633
Adamska M, et al., Doubleridge, a mouse mutant with defective compaction of the apical ectodermal ridge and normal dorsal-ventral patterning of the limb. Dev Biol. 2003 Mar 15;255(2):350-62
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5499820
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
8A:
embryonic day 11.5
(more  )
Specimen
8A:
(close  )
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Genetic Background:
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either: C57BL/6J or (SJL/J x C57BL/6J)F1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb |
Present |
Regionally restricted |
8A
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Expression was detected in two areas below the posterior end of the apical ectodermal ridge.
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Specimen
8B:
embryonic day 11.5
(more )
Note: Double labeled: purple - Shh; pink - Fgf8 (assay MGI:5499813).
Specimen
8B:
(close )
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Genetic Background:
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either: C57BL/6J or (SJL/J x C57BL/6J)F1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Shh; pink - Fgf8 (assay MGI:5499813).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb |
Present |
Regionally restricted |
8B
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Expression was detected in two areas below the posterior end of the apical ectodermal ridge.
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Analysis Tools
