Antibody:	clone 39.4D5
Assay notes:	The antibody used in this assay also recognizes a related gene see MGI:5496009.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted		Expression was detected in motor neurons in the ventrolateral column. Graphed in Fig. 4A.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted		The position and appearance of motor neurons appeared indistinguishable from the control littermates. Graphed in Fig. 4A.

Structure	Level	Pattern	Image	Note
TS19: neural tube mantle layer	Present	Regionally restricted		Expression was detected in motor neurons in the ventrolateral column. Graphed in Fig. 4A.

Structure	Level	Pattern	Image	Note
TS19: neural tube mantle layer	Present	Regionally restricted		The position and appearance of motor neurons appeared indistinguishable from the control littermates. Graphed in Fig. 4A.

Structure	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	Present	Regionally restricted	1A	Expression was detected in motor neurons in the ventrolateral column. Graphed in Fig. 4A.
TS20: dorsal root ganglion	Present	Regionally restricted	1A	Expression was detected in the sensory neurons.

Structure	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	Present	Regionally restricted	1B	The position and appearance of motor neurons appeared indistinguishable from the control littermates. Graphed in Fig. 4A.

Structure	Level	Pattern	Image	Note
TS21: spinal cord mantle layer	Present	Regionally restricted	1C	Expression was detected in motor neurons in the medial and lateral median motor columns and in the visceral motor column. The graph in fig. 4A showed a decrease over time.

Structure	Level	Pattern	Image	Note
TS21: spinal cord mantle layer	Present	Single cells	1D	Expression was in motor neurons. Expression in the visceral motor column was similar to wild type. Expression in the medial and lateral median motor columns of the mutant was significantly lower than in wild type. Graphed in Fig. 4A, 4C and 4D.

Structure	Level	Pattern	Image	Note
TS22: spinal cord mantle layer	Present	Regionally restricted		Expression was detected in motor neurons in the medial and lateral median motor columns and in the visceral motor column. The graph in fig. 4A showed a decrease over time.

Structure	Level	Pattern	Image	Note
TS22: spinal cord mantle layer	Present	Single cells		Expression was in motor neurons. Expression in the visceral motor column was similar to wild type. Expression in the medial and lateral median motor columns of the mutant was significantly lower than in wild type. Graphed in Fig. 4A, 4C and 4D.

Structure	Level	Pattern	Image	Note
TS23: spinal cord mantle layer	Present	Regionally restricted	1E	Expression was detected in motor neurons in the medial and lateral median motor columns and in the visceral motor column. The graph in fig. 4A showed a decrease over time.

Structure	Level	Pattern	Image	Note
TS23: spinal cord mantle layer	Present	Single cells	1F	Expression was in motor neurons. Expression in the visceral motor column was similar to wild type. Expression in the medial and lateral median motor columns of the mutant was significantly lower than in wild type. Graphed in Fig. 4A, 4C and 4D.

Structure	Level	Pattern	Image	Note
TS24: spinal cord mantle layer	Present	Regionally restricted		Expression was detected in motor neurons in the medial and lateral median motor columns and in the visceral motor column. The graph in fig. 4A showed a decrease over time.

Structure	Level	Pattern	Image	Note
TS24: spinal cord mantle layer	Present	Single cells		Expression was in motor neurons. Expression in the visceral motor column was similar to wild type. No expression was in the medial and lateral median motor columns of the mutant. Graphed in Fig. 4A, 4C and 4D.

Structure	Level	Pattern	Image	Note
TS25: spinal cord mantle layer	Present	Regionally restricted	1G	Expression was detected in motor neurons in the medial and lateral median motor columns and in the visceral motor column. The graph in fig. 4A showed a decrease over time.

Structure	Level	Pattern	Image	Note
TS25: spinal cord mantle layer	Present	Single cells	1H	Expression was in motor neurons. Expression in the visceral motor column was similar to wild type. No expression was in the medial and lateral median motor columns of the mutant. Graphed in Fig. 4A, 4C and 4D.

Structure	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	Present	Regionally restricted	2A	Expression was detected in somatic motor neurons in the lateral motor column. Expression level decreased from E12.5-14.5. Graphed in Fig. 4E.

Structure	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	Present	Regionally restricted	2B	Expression was detected in somatic motor neurons in the lateral motor column. Expression level decreased by 91% from E12.5-14.5. Graphed in Fig. 4E.

Structure	Level	Pattern	Image	Note
TS21: spinal cord mantle layer	Present	Regionally restricted	2C	Expression was detected in somatic motor neurons in the lateral motor column. Expression level decreased from E12.5-14.5. Graphed in Fig. 4E.

Structure	Level	Pattern	Image	Note
TS21: spinal cord mantle layer	Present	Single cells	2D	Expression was detected in somatic motor neurons in the lateral motor column. Expression level decreased by 91% from E12.5-14.5. Graphed in Fig. 4E.

Structure	Level	Pattern	Image	Note
TS22: spinal cord mantle layer	Present	Regionally restricted	2E	Expression was detected in somatic motor neurons in the lateral motor column. Expression level decreased from E12.5-14.5. Graphed in Fig. 4E.

Structure	Level	Pattern	Image	Note
TS22: spinal cord mantle layer	Present	Single cells	2F	Expression was detected in somatic motor neurons in the lateral motor column. Expression level decreased by 91% from E12.5-14.5. Graphed in Fig. 4E.