Gene Expression Data
Assay Details
Assay
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Reference:
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J:104896
Cho JH, et al., Preferential posterior cerebellum defect in BETA2/NeuroD1 knockout mice is the result of differential expression of BETA2/NeuroD1 along anterior-posterior axis. Dev Biol. 2006 Feb 1;290(1):125-38
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5491696
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Gene symbol:
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Neurod1
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Gene name:
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neurogenic differentiation 1
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Antibody:
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anti-Neurod1 (N-19; sc-1084)
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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This antibody detects a short peptide from the initial N-terminal part of NeuroD1 protein, for which the coding region is not deleted in the
this knockout mouse.
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Results
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Specimen
6A:
embryonic day 17.5
(more )
Note: Double labeled: green - Neurod1; red - Ntrk3 (TrkC; assay MGI:5491701).
Specimen
6A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Neurod1; red - Ntrk3 (TrkC; assay MGI:5491701).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: cerebellum |
Present |
Regionally restricted |
6Aa
,
6Aa'
,
6Aa''
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Expression is in a posterior to anterior gradient in the inner half of the external granule layer.
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Specimen
6B:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd * 129X1/SvJ
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Mutant Allele(s):
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Neurod1tm1Mjts/Neurod1tm1Mjts
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Neurod1; red - Ntrk3 (TrkC; assay MGI:5491701).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: cerebellum |
Present |
Regionally restricted |
6Ab'
,
6Ab''
,
6Ab
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Expression is in a posterior to anterior gradient in the inner half of the external granule layer.
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