Gene Expression Data
Assay Details
Assay
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Reference:
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J:91339
Takebayashi S, et al., Expression of beta-catenin in developing auditory epithelia of mice. Acta Otolaryngol Suppl. 2004;(551):18-21
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5478595
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Gene symbol:
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Ctnnb1
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Gene name:
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catenin beta 1
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Antibody:
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Anti-Beta-catenin
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
1A:
embryonic day 12.0
(more )
Note: Double labeled: green - Ctnnb1; red - Mki67 (assay MGI:5478594).
Specimen
1A:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Ctnnb1; red - Mki67 (assay MGI:5478594).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cochlear duct epithelium |
Strong |
Regionally restricted |
1A
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Intense expression was detected in the apical surface of the auditory epithelia. There was no difference in expression patterns observed among epithelial regions.
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Specimen
1B:
embryonic day 13.0
(more )
Note: Double labeled: green - Ctnnb1; red - Mki67 (assay MGI:5478594).
Specimen
1B:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Ctnnb1; red - Mki67 (assay MGI:5478594).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cochlear duct epithelium |
Present |
Regionally restricted |
1B
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Expression was detected in the apical surface of all epithelial cells. In the immature organ of Corti cells expression was only in the apical surface. Expression that overlapped with Ki-67 was found both in the apical and basal portion.
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Specimen
1C:
embryonic day 15.0
(more )
Note: Double labeled: green - Ctnnb1; red - Mki67 (assay MGI:5478594).
Specimen
1C:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Ctnnb1; red - Mki67 (assay MGI:5478594).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cochlea epithelium |
Present |
Regionally restricted |
1C
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Expression was limited to the apical surface of epithelial cells. Only weak expression was in the basal portion of the auditory epithelium. Weak expression was also noted in the organ of Corti.
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Specimen
2A:
embryonic day 17.0
(more )
Note: Double labeled: green - Ctnnb1; red - Myo7a (assay MGI:5478596).
Specimen
2A:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Ctnnb1; red - Myo7a (assay MGI:5478596).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: organ of Corti |
Strong |
Regionally restricted |
2A
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Expression was strong in the apical portion of the outer hair cells.
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TS25: greater epithelial ridge |
Strong |
Regionally restricted |
2A
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Expression was strong in the apical portion.
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TS25: cochlea epithelium |
Weak |
Regionally restricted |
2A
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Weak expression was detected in the apical portion between the outer hair cells and the greater epithelial ridge.
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Specimen
2B:
postnatal day 7
(more )
Note: Double labeled: green - Ctnnb1; red - Myo7a (assay MGI:5478596).
Specimen
2B:
(close )
Genetic Background:
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ICR
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Ctnnb1; red - Myo7a (assay MGI:5478596).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cochlea |
Strong |
Regionally restricted |
2B
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Strong expression was detected in the apical portion of the outer hair cells and greater epithelial ridge, but not inner hair cells. Outer hair cell distribution corresponded to the location of adherens junctions. Expression was in pillar cells.
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