Antibody:	anti-CD31 (2H8 MAB1398Z)
Detection system:	Secondary antibody
Assay notes:	The AMeX method was used to process the embryos.

Structure	Level	Pattern	Image	Note
TS22: arterial system	Present	Regionally restricted	2B	Expression was detected in endothelial cells.
TS22: blood vessel	Present	Regionally restricted	2B	Expression was detected in endothelial cells.
TS22: venous system	Present	Regionally restricted	2B	Expression was detected in endothelial cells.
TS22: lymphatic system	Weak	Regionally restricted	2B	Expression was detected in endothelial cells.

Structure	Level	Pattern	Image	Note
TS22: arterial system	Present	Regionally restricted	2C	Expression was detected in endothelial cells.
TS22: blood vessel	Present	Regionally restricted	2C	Expression was detected in endothelial cells.
TS22: lymphatic system	Weak	Regionally restricted	2C	Expression was detected in endothelial cells.

Structure	Level	Pattern	Image	Note
TS22: blood vessel	Present	Regionally restricted	3E	Expression was detected in endothelial cells.
TS22: lymphatic system	Weak	Regionally restricted	3E	Expression was detected in endothelial cells.

Structure	Level	Pattern	Image	Note
TS22: blood vessel	Present	Regionally restricted	3F	Expression was detected in endothelial cells. Blood vessels appear relatively unaffected in the mutant.
TS22: lymphatic system	Weak	Regionally restricted	3F	Expression was detected in endothelial cells. Lymphatic vessels appear distended.

Structure	Level	Pattern	Image	Note
TS22: blood vessel	Present	Regionally restricted	4A , 4E	Expression was detected in endothelial cells in back skin.
TS22: lymphatic system	Weak	Regionally restricted	4A , 4E	Expression was detected in endothelial cells in back skin.

Structure	Level	Pattern	Image	Note
TS22: blood vessel	Present	Regionally restricted	4B , 4F	Blood vessels were relatively unaffected in the mutant.
TS22: lymphatic system	Weak	Regionally restricted	4B , 4F	Lymphatic vessels were disorganized and partly distended in the mutant.

Structure	Level	Pattern	Image	Note
TS23: blood vessel	Present	Regionally restricted	4G , 4K	Expression was detected in endothelial cells in back skin.
TS23: lymphatic system	Weak	Regionally restricted	4K , 4G	Expression was detected in endothelial cells in back skin.

Structure	Level	Pattern	Image	Note
TS23: blood vessel	Present	Regionally restricted	4L , 4H	Blood vessels were relatively unaffected in the mutant.
TS23: lymphatic system	Weak	Regionally restricted	4H , 4L	Collecting vessels were tortuous and distended in the mutant. Isolated ball-like structures of numerous lymphatic endothelial cells - lymphatic islands - were detected in the mutant.

Structure	Level	Pattern	Image	Note
TS24: blood vessel	Present	Regionally restricted	S1A , S1E	Expression was detected in endothelial cells in back skin.
TS24: lymphatic system	Weak	Regionally restricted	S1A , S1E	Expression was detected in endothelial cells in back skin.

Structure	Level	Pattern	Image	Note
TS24: blood vessel	Present	Regionally restricted	S1F , S1B	Blood vessels were relatively unaffected in the mutant.
TS24: lymphatic system	Weak	Regionally restricted	S1F , S1B	The lymphatic network was defective and lymphatic islands were detected in the mutant.