Gene Expression Data
Assay Details
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Assay
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Reference:
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J:134562
Trapp ML, et al., Defects in ciliary localization of Nek8 is associated with cystogenesis. Pediatr Nephrol. 2008 Mar;23(3):377-87
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5475469
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Gene symbol:
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Nek8
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Gene name:
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NIMA (never in mitosis gene a)-related expressed kinase 8
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Antibody:
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anti-Nek8
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
2b:
embryonic day 18.5
(more  )
Note: Double labeled: green - Nek8; red - Calb1 (assay MGI:5475489).
Specimen
2b:
(close  )
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Genetic Background:
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involves: 129 * CD-1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Nek8; red - Calb1 (assay MGI:5475489).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: renal tubule |
Present |
Regionally restricted |
2b merge
,
2b left
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Expressed in the luminal cilia of the medullary tubule.
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| TS26: collecting duct |
Present |
Regionally restricted |
2b merge
,
2b left
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Expressed in the cilia of the developing and maturing collecting ducts.
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Specimen
5a:
postnatal day 7
(more )
Specimen
5a:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: renal tubule |
Present |
Regionally restricted |
5a
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Expressed in the luminal cilia of the medullary tubule.
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Specimen
5b:
postnatal day 7; Nek8jck/Nek8 +
(more )
Specimen
5b:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Nek8jck/Nek8+
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: renal tubule |
Present |
Regionally restricted |
5b
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Expressed in the majority of luminal cilia within a given tubule.
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Specimen
5c:
postnatal day 7; Nek8jck/ Nek8jck
(more )
Note: The specimen is a non-cystic ciliated tubule.
Specimen
5c:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Nek8jck/Nek8jck
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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The specimen is a non-cystic ciliated tubule.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: renal tubule |
Present |
Regionally restricted |
5c
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Expressed in fewer cilia within a given tubule.
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Specimen
5d:
postnatal day 7; Nek8jck/ Nek8jck
(more )
Note: The specimen is a cystic ciliated tubule.
Specimen
5d:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Nek8jck/Nek8jck
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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The specimen is a cystic ciliated tubule.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: renal tubule |
Present |
Regionally restricted |
5d
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Expressed in fewer cilia within a given tubule.
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Specimen
S1a:
embryonic day 18.5
(more )
Note: Double labeled: green - Nek8; red - Ncam1 (assay MGI:5475503).
Specimen
S1a:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Nek8; red - Ncam1 (assay MGI:5475503).
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Analysis Tools