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Gene Expression Data
Assay Details
Assay
Reference: J:138322 Smith L, et al., uPARAP expression during murine lung development. Gene Expr Patterns. 2008 Sep;8(7-8):486-93
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5449220
Gene symbol: Col1a2
Gene name: collagen, type I, alpha 2
Antibody: anti-collagen I
Detection system: Secondary antibody coupled to Alexa Fluor 568
Assay notes: The specificity of the antibody used was not detailed; Col1a1 (MGI:5449218) has also been annotated.
Results
Specimen 5A: embryonic day 15.5 (more )
Note: Double labeled: red - Col1a1 and Col1a2; green - Mrc2 (uPARAP; assay MGI:5449217).
Structure Level Pattern Image Note
TS23: lung epithelium Present 5A Expressed around developing airways, with no overlapping staining for uPARAP.

Specimen 5B: embryonic day 15.5 (more )
Note: Double labeled: red - Col1a1 and Col1a2; green - Mrc2 (uPARAP; assay MGI:5449217).
Structure Level Pattern Image Note
TS23: lung epithelium Strong 5B Expressed around developing airways, with no overlapping staining for uPARAP.

Specimen 5C: embryonic day 17.5 (more )
Note: Double labeled: red - Col1a1 and Col1a2; green - Mrc2 (uPARAP; assay MGI:5449217).
Structure Level Pattern Image Note
TS25: lung mesenchyme Present Scattered 5C Occasional mesenchymal expression.
TS25: lung vascular element Present 5C With minimal uPARAP coexpression.
TS25: lung epithelium Present 5C

Specimen 5D: embryonic day 17.5 (more )
Note: Double labeled: red - Col1a1 and Col1a2; green - Mrc2 (uPARAP; assay MGI:5449217).
Structure Level Pattern Image Note
TS25: lung mesenchyme Present Scattered 5D Occasional mesenchymal expression.
TS25: lung vascular element Present 5D With minimal uPARAP coexpression.
TS25: lung epithelium Present 5D

Specimen 5E: postnatal day 3 (more )
Note: Double labeled: red - Col1a1 and Col1a2; green - Mrc2 (uPARAP; assay MGI:5449217).
Structure Level Pattern Image Note
TS27: alveolus epithelium Present 5E Expressed along the septal tips, with no overlapping staining for uPARAP.

Specimen 5F: postnatal day 3 (more )
Note: Double labeled: red - Col1a1 and Col1a2; green - Mrc2 (uPARAP; assay MGI:5449217).
Structure Level Pattern Image Note
TS27: alveolus epithelium Present 5F Expressed along the septal tips, with no overlapping staining for uPARAP.

Specimen 5G: postnatal day 10 (more )
Note: Double labeled: red - Col1a1 and Col1a2; green - Mrc2 (uPARAP; assay MGI:5449217).
Structure Level Pattern Image Note
TS28: alveolus epithelium Present 5G Expressed along the septal tips, with no overlapping staining for uPARAP.

Specimen 5H: postnatal day 10 (more )
Note: Double labeled: red - Col1a1 and Col1a2; green - Mrc2 (uPARAP; assay MGI:5449217).
Structure Level Pattern Image Note
TS28: alveolus epithelium Present 5H Expressed along the septal tips, with no overlapping staining for uPARAP.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory