Gene Expression Data
Assay Details
Assay
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Reference:
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J:123586
Fitzgerald DP, et al., Neogenin is expressed on neurogenic and gliogenic progenitors in the embryonic and adult central nervous system. Gene Expr Patterns. 2007 Aug;7(7):784-92
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5440298
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Gene symbol:
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Pcna
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Gene name:
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proliferating cell nuclear antigen
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Antibody:
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Anti-PCNA
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Detection system:
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Secondary antibody coupled to Alexa Fluor 568
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Results
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Specimen
1B/C:
embryonic day 14.5
(more )
Note: Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Specimen
1B/C:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
|
Sex:
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Not Specified
|
Type:
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section
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Fixation:
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Other - See Notes
|
Embedding:
|
Cryosection
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Note:
|
Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hippocampus |
Present |
Regionally restricted |
1B
,
1C
|
Expression was detected in nuclei of S-phase dividing cells at the basal surface of the ventricular zone.
|
TS22: telencephalon septum |
Present |
Regionally restricted |
1B
,
1C
|
Expression was detected in nuclei of S-phase dividing cells at the basal surface of the ventricular zone.
|
Specimen
1H/I:
embryonic day 14.5
(more )
Note: Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Specimen
1H/I:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: olfactory cortex ventricular layer |
Present |
Regionally restricted |
1H
,
1I
|
Expression was detected in nuclei of S-phase dividing cells at the basal surface of the ventricular zone.
|
TS22: telencephalon septum |
Present |
Regionally restricted |
1H
,
1I
|
Expression was detected in nuclei of S-phase dividing cells at the basal surface of the ventricular zone.
|
Specimen
2B:
embryonic day 12.5
(more )
Note: Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Specimen
2B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: midbrain |
Present |
Regionally restricted |
2B
|
Expression was detected in dividing cells.
|
Specimen
2D:
embryonic day 14.5
(more )
Note: Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Specimen
2D:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: midbrain ventricular layer |
Present |
Regionally restricted |
2D
|
Expression was detected in dividing cells in the dorsal midbrain ventricular zone.
|
Specimen
2I:
embryonic day 14.5
(more )
Note: Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Specimen
2I:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
|
Specimen
3B/C:
embryonic day 17.5
(more )
Note: Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Specimen
3B/C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
|
|