Gene Expression Data
Assay Details
Assay
|
Reference:
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J:179032
Sharlin DS, et al., Developmental and cell-specific expression of thyroid hormone transporters in the mouse cochlea. Endocrinology. 2011 Dec;152(12):5053-64
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5311189
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Gene symbol:
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Slc7a5
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Gene name:
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solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
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Antibody:
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anti-LAT1 (Q20)
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
3B:
postnatal day 7
(more )
Note: Double labeled: red - Slc7a5; green - Pecam1 (assay MGI:5311190).
Specimen
3B:
(close )
Genetic Background:
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C57BL/6J
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Age:
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postnatal day 7
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
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2% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
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Double labeled: red - Slc7a5; green - Pecam1 (assay MGI:5311190).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: blood vessel |
Present |
Regionally restricted |
3B Lat1
,
3B Lat1 CD31 DAPI
,
3B Lat1 CD31
|
Protein almost exclusively localized to CD31-positive blood vessels in the spiral ligament and stria vascularis.
|
Specimen
3D:
postnatal day 3
(more )
Note: Double labeled: red - Slc7a5; green - Myo7a (assay MGI:5311371).
Specimen
3D:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Slc7a5; green - Myo7a (assay MGI:5311371).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: organ of Corti |
Present |
Regionally restricted |
3D
|
Signal localized to the cell membrane of hair cells and Deiters cells.
|
TS27: cochlear duct |
Present |
Regionally restricted |
3D
|
Positive in spiral vessel.
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Specimen
3F:
postnatal day 1
(more )
Note: Double labeled: red - Slc7a5; green - Pecam1 (assay MGI:5311190).
Specimen
3F:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Slc7a5; green - Pecam1 (assay MGI:5311190).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: cochlear ganglion |
Weak |
Regionally restricted |
3F
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Weak signal was detected in neuronal soma.
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TS27: blood vessel |
Present |
|
3F
|
Signal was detected in CD31-positive blood vessels.
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Specimen
3H:
postnatal day 7
(more )
Note: Double labeled: red - Slc7a5; green - Pecam1 (assay MGI:5311190).
Specimen
3H:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Slc7a5; green - Pecam1 (assay MGI:5311190).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spiral vessel |
Present |
|
3H Lat1
,
3H Lat1 CD31 DAPI
,
3H Lat1 CD31
|
Signal overlapped with CD31.
|
Specimen
S1B:
postnatal day 7
(more )
Specimen
S1B:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
postnatal day 7
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|