Nefm Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:4501 Mansour SL, et al., Mice homozygous for a targeted disruption of the proto-oncogene int-2 have developmental defects in the tail and inner ear. Development. 1993 Jan;117(1):13-28
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5050134
Gene symbol:	Nefm
Gene name:	neurofilament, medium polypeptide

Antibody:	anti-neurofilament clone 2H3
Assay notes:	Embryos (21-25 somites) were fixed in 2% formaldehyde for 6 minutes.

Results

6E ©

Specimen 6E: embryonic day 9.5; Fgf3^tm2Mrc/Fgf3⁺ (more

)
Note: Double labeled: brown - Nefm; blue - Fgf3 reporter (assay MGI:5050137).

Structure	Level	Pattern	Image	Note
TS15: cranial preganglion	Present		6E

6F ©

Specimen 6F: embryonic day 9.5; Fgf3^tm2Mrc/Fgf3^tm1Mrc (more

)
Note: Double labeled: brown - Nefm; blue - Fgf3 reporter (assay MGI:5050137).

Structure	Level	Pattern	Image	Note
TS15: cranial preganglion	Present		6F

6G ©

Specimen 6G: embryonic day 9.5; Fgf3^tm2Mrc/Fgf3^tm1Mrc (more

)
Note: Double labeled: brown - Nefm; blue - Fgf3 reporter (assay MGI:5050137).

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present		6G	A coronal section taken through the ventral region of the otocyst.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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