Gene Expression Data
Assay Details
Assay
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Reference:
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J:157799
Acharya M, et al., Human PRKC apoptosis WT1 regulator is a novel PITX2-interacting protein that regulates PITX2 transcriptional activity in ocular cells. J Biol Chem. 2009 Dec 11;284(50):34829-38
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4455009
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Gene symbol:
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Pitx2
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Gene name:
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paired-like homeodomain transcription factor 2
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Antibody:
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Pitx2 antibody
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Detection system:
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Secondary antibody coupled to biotin
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Assay notes:
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Signals were detected using tyramide
signal amplification kits.
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Results
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Specimen
6A,C:
embryonic day 12.5
(more )
Note: Double labeled: green - Pitx2; red - Pawr (assay MGI:4455010).
Specimen
6A,C:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Pitx2; red - Pawr (assay MGI:4455010).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: eye |
Present |
Regionally restricted |
6C
,
6A
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Expression is throughout the periocular mesenchyme.
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Specimen
6D,F:
embryonic day 14.5
(more )
Note: Double labeled: green - Pitx2; red - Pawr (assay MGI:4455010).
Specimen
6D,F:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Pitx2; red - Pawr (assay MGI:4455010).
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Specimen
6G,I:
embryonic day 18.5
(more )
Note: Double labeled: green - Pitx2; red - Pawr (assay MGI:4455010).
Specimen
6G,I:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Note:
|
Double labeled: green - Pitx2; red - Pawr (assay MGI:4455010).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: eye |
Present |
Regionally restricted |
6G
,
6I
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Expression is in the iridocorneal angle.
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Specimen
6J,L:
embryonic day 18.5
(more )
Note: Double labeled: green - Pitx2; red - Pawr (assay MGI:4455010).
Specimen
6J,L:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Pitx2; red - Pawr (assay MGI:4455010).
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Specimen
S2:
embryonic day 14.5
(more )
Note: Double labeled: green - Pitx2; red - Pawr (assay MGI:4455010).
Specimen
S2:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Pitx2; red - Pawr (assay MGI:4455010).
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