Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type:	RNA in situ
MGI Accession ID:	MGI:4452070
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	MGC clone 69975
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

3b ©

Specimen 3b: embryonic day 12.5 (more

)
Note: Double labeled: blue - Shh; brown - Arx (assay MGI:4452072).

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present		3b

6a ©

Specimen 6a: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	6a	Expression is present in the basal hypothalamus and zona limitans intrathalamica.
TS17: telencephalon	Present	Regionally restricted	6a	Expression is present in the basal telencephalon.

6b ©

Specimen 6b: embryonic day 10.5; Shh^tm2Amc/Shh^tm2Amc, Tg(Nkx2-1-cre)2Sand/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	6b	There is no expression in the basal hypothalamus, although expression persists in more posterior regions and the zona limitans intrathalmica.
TS17: telencephalon	Absent		6b	There is no expression in the basal telencephalon.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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