About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type: RNA in situ
MGI Accession ID: MGI:4450786
Gene symbol: Nkx6-2
Gene name: NK6 homeobox 2
Probe: UI-M-BH3-awd-f-01-0-UI.s1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 3m: embryonic day 12.5 (more )
Note: Double labeled: blue - Nkx6-2; brown - Arx (assay MGI:4452072).
Structure Level Pattern Image Note
TS20: hypothalamus Present Regionally restricted 3m Expression is present in a domain of ventral anterior hypothalamic neuroepithelium.
Specimen S5a: embryonic day 11.5 (more )
Note: Double labeled: blue - Nkx6-2; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S5_a Expression is present in the anterioventral hypothalamus.
Specimen S5b: embryonic day 12.5 (more )
Note: Double labeled: blue - Nkx6-2; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS20: hypothalamus Present Regionally restricted S5_b Expression is present in the anterioventral hypothalamus.
Specimen S5c: embryonic day 14.5 (more )
Note: Double labeled: blue - Nkx6-2; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS22: hypothalamus Present Regionally restricted S5_c Expression is present in the anterioventral hypothalamus.
Specimen S5d: embryonic day 16.5 (more )
Note: Double labeled: blue - Nkx6-2; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS24: hypothalamus Present Regionally restricted S5_d Expression is present in the anterioventral hypothalamus.
Specimen S6i: embryonic day 12.5 (more )
Note: Double labeled: blue - Nkx6-2; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS20: hypothalamus Present Regionally restricted S6_i Expression is present in the anterioventral hypothalamus.
Specimen S6j: embryonic day 12.5; Shhtm2Amc/Shhtm2Amc, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant. Double labeled: blue - Nkx6-2; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS20: hypothalamus Absent S6_j Anterioventral hypothalamus-specific expression is absent from the mutant.
Specimen Nkx6-2 - E11.5: embryonic day 11.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS19: diencephalon lateral wall mantle layer Ambiguous NKX6.2(D)+SHH(F)-E11.5-coronal , NKX6.2(D)+SHH(F)-E11.5-sagittal , Nkx6.2-Shh E11.5_coronal-2 Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus, prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: diencephalon lateral wall ventricular layer Strong Non-Uniform Nkx6.2-Shh E11.5_coronal-2 , NKX6.2(D)+SHH(F)-E11.5-coronal , NKX6.2(D)+SHH(F)-E11.5-sagittal There is strong staining in the VZ of the AVH. Staining is either absent or ubiquitous in the VZ of the dorsal thalamus, prethalamus, dorsal & ventral POAs, the PVH, VMH & ARH nuclei, anterior & posterior IDs, premam, mam & supramam zones & the TT.
TS19: telencephalon mantle layer Ambiguous NKX6.2(D)+SHH(F)-E11.5-sagittal , NKX6.2(D)+SHH(F)-E11.5-coronal , Nkx6.2-Shh E11.5_coronal-2 Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer Strong Non-Uniform NKX6.2(D)+SHH(F)-E11.5-sagittal , Nkx6.2-Shh E11.5_coronal-2 , NKX6.2(D)+SHH(F)-E11.5-coronal There is strong staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
Specimen Nkx6-2 - E12.5: embryonic day 12.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS20: cerebral cortex intermediate zone Ambiguous NKX6.2(D)+SHH(F)-E12.5-coronal , Nkx6.2-Shh E12.5_coronal-2 , NKX6.2(D)+SHH(F)-E12.5-sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer Ambiguous Nkx6.2-Shh E12.5_coronal-2 , NKX6.2(D)+SHH(F)-E12.5-sagittal , NKX6.2(D)+SHH(F)-E12.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate Ambiguous Nkx6.2-Shh E12.5_coronal-2 , NKX6.2(D)+SHH(F)-E12.5-sagittal , NKX6.2(D)+SHH(F)-E12.5-coronal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer Ambiguous Nkx6.2-Shh E12.5_coronal-2 , NKX6.2(D)+SHH(F)-E12.5-coronal , NKX6.2(D)+SHH(F)-E12.5-sagittal Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer Ambiguous Nkx6.2-Shh E12.5_coronal-2 , NKX6.2(D)+SHH(F)-E12.5-sagittal , NKX6.2(D)+SHH(F)-E12.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence Ambiguous NKX6.2(D)+SHH(F)-E12.5-coronal , Nkx6.2-Shh E12.5_coronal-2 , NKX6.2(D)+SHH(F)-E12.5-sagittal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer Ambiguous NKX6.2(D)+SHH(F)-E12.5-sagittal , Nkx6.2-Shh E12.5_coronal-2 , NKX6.2(D)+SHH(F)-E12.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer Strong Non-Uniform NKX6.2(D)+SHH(F)-E12.5-coronal , Nkx6.2-Shh E12.5_coronal-2 , NKX6.2(D)+SHH(F)-E12.5-sagittal There is strong staining in the ventricular zone of the AVH. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei and anterior and posterior IDs.
TS20: thalamus mantle layer Ambiguous Nkx6.2-Shh E12.5_coronal-2 , NKX6.2(D)+SHH(F)-E12.5-coronal , NKX6.2(D)+SHH(F)-E12.5-sagittal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer Ambiguous NKX6.2(D)+SHH(F)-E12.5-coronal , Nkx6.2-Shh E12.5_coronal-2 , NKX6.2(D)+SHH(F)-E12.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
Specimen Nkx6-2 - E14.5: embryonic day 14.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Ambiguous NKX6.2(D)+SHH(F)-E14.5-sagittal , NKX6.2(D)+SHH(F)-E14.5-coronal , Nkx6.2-Shh E14.5_coronal-2 Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer Ambiguous NKX6.2(D)+SHH(F)-E14.5-sagittal , Nkx6.2-Shh E14.5_coronal-2 , NKX6.2(D)+SHH(F)-E14.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate Ambiguous NKX6.2(D)+SHH(F)-E14.5-coronal , NKX6.2(D)+SHH(F)-E14.5-sagittal , Nkx6.2-Shh E14.5_coronal-2 Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer Ambiguous Nkx6.2-Shh E14.5_coronal-2 , NKX6.2(D)+SHH(F)-E14.5-sagittal , NKX6.2(D)+SHH(F)-E14.5-coronal Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer Ambiguous Nkx6.2-Shh E14.5_coronal-2 , NKX6.2(D)+SHH(F)-E14.5-coronal , NKX6.2(D)+SHH(F)-E14.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence Strong Non-Uniform NKX6.2(D)+SHH(F)-E14.5-sagittal , Nkx6.2-Shh E14.5_coronal-2 , NKX6.2(D)+SHH(F)-E14.5-coronal There is strong staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS22: hypothalamus mantle layer Ambiguous NKX6.2(D)+SHH(F)-E14.5-sagittal , Nkx6.2-Shh E14.5_coronal-2 , NKX6.2(D)+SHH(F)-E14.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer Strong Non-Uniform Nkx6.2-Shh E14.5_coronal-2 , NKX6.2(D)+SHH(F)-E14.5-sagittal , NKX6.2(D)+SHH(F)-E14.5-coronal There is strong staining in the ventricular zone of the AVH. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei and anterior and posterior IDs.
TS22: thalamus mantle layer Ambiguous Nkx6.2-Shh E14.5_coronal-2 , NKX6.2(D)+SHH(F)-E14.5-coronal , NKX6.2(D)+SHH(F)-E14.5-sagittal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer Ambiguous NKX6.2(D)+SHH(F)-E14.5-sagittal , NKX6.2(D)+SHH(F)-E14.5-coronal , Nkx6.2-Shh E14.5_coronal-2 Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
Specimen Nkx6-2 - E16.5: embryonic day 16.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Ambiguous NKX6.2(D)+SHH(F)-E16.5-sagittal , NKX6.2(D)+SHH(F)-E16.5-coronal , Nkx6.2-Shh E16.5_coronal-2 Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer Ambiguous NKX6.2(D)+SHH(F)-E16.5-sagittal , Nkx6.2-Shh E16.5_coronal-2 , NKX6.2(D)+SHH(F)-E16.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate Ambiguous NKX6.2(D)+SHH(F)-E16.5-sagittal , NKX6.2(D)+SHH(F)-E16.5-coronal , Nkx6.2-Shh E16.5_coronal-2 Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer Ambiguous Nkx6.2-Shh E16.5_coronal-2 , NKX6.2(D)+SHH(F)-E16.5-sagittal , NKX6.2(D)+SHH(F)-E16.5-coronal Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer Ambiguous Nkx6.2-Shh E16.5_coronal-2 , NKX6.2(D)+SHH(F)-E16.5-sagittal , NKX6.2(D)+SHH(F)-E16.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence Ambiguous NKX6.2(D)+SHH(F)-E16.5-coronal , Nkx6.2-Shh E16.5_coronal-2 , NKX6.2(D)+SHH(F)-E16.5-sagittal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer Ambiguous NKX6.2(D)+SHH(F)-E16.5-coronal , Nkx6.2-Shh E16.5_coronal-2 , NKX6.2(D)+SHH(F)-E16.5-sagittal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer Strong Non-Uniform Nkx6.2-Shh E16.5_coronal-2 , NKX6.2(D)+SHH(F)-E16.5-sagittal , NKX6.2(D)+SHH(F)-E16.5-coronal There is strong staining in the ventricular zone of the AVH and the DAH. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei and the PH.
TS24: thalamus mantle layer Ambiguous NKX6.2(D)+SHH(F)-E16.5-sagittal , NKX6.2(D)+SHH(F)-E16.5-coronal , Nkx6.2-Shh E16.5_coronal-2 Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer Ambiguous Nkx6.2-Shh E16.5_coronal-2 , NKX6.2(D)+SHH(F)-E16.5-sagittal , NKX6.2(D)+SHH(F)-E16.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/14/2024
MGI 6.23 		The Jackson Laboratory