Pou5f1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:140465 Guo G, et al., Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst. Dev Cell. 2010 Apr 20;18(4):675-85
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:4440941
Gene symbol:	Pou5f1
Gene name:	POU domain, class 5, transcription factor 1

Antibody:	anti-Pou5f1
Detection system:	Secondary antibody
Assay notes:	Embryos were fixed in 2.5% paraformaldehyde.

Results

Specimen S1B -- second row: embryonic day 3.5 (more

)
Note: Double labeled: red - Pou5f1; green - Id2 (assay MGI:4440939).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		S1B Pou5f1 2nd row , S1B Merged 2nd row	Expression is present in the epiblast.

Specimen S1B -- fourth row: embryonic day 3.5 (more

)
Note: Double labeled: green - Pou5f1; red - Cebpa (assay MGI:4440942).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		S1B Pou5f1 4th row , S1B Merged 4th row	Expression is present in the epiblast.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/18/2025 MGI 6.24