Gene Expression Data
Assay Details
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Assay
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Reference:
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J:147173
Sultana DA, et al., Gene expression profile of the third pharyngeal pouch reveals role of mesenchymal MafB in embryonic thymus development. Blood. 2009 Mar 26;113(13):2976-87
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3848220
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Gene symbol:
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Ccl21b
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Gene name:
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C-C motif chemokine ligand 21B (leucine)
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Antibody:
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Anti-CCL21
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Assay notes:
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Sections were fixed prior to staining with either acetone or paraformaldehyde. The specificity of the antibody used was not detailed; other family members have been annotated (see assays MGI:3848218 and MGI:3848221).
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Results
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Specimen
8B/C Wt #1:
embryonic day 11.5
(more  )
Specimen
8B/C Wt #1:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
8H/I Wt #2:
embryonic day 11.5
(more )
Specimen
8H/I Wt #2:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
8N/O MafB-KO #1:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Mutant Allele(s):
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Mafbtm1Jeng/Mafbtm1Jeng
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: 3rd branchial pouch |
Weak |
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8N
,
8O
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Expression was less detectable in the mutant compared to wild type.
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Specimen
8T/U MafB-KO #2:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Mutant Allele(s):
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Mafbtm1Jeng/Mafbtm1Jeng
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: 3rd branchial pouch |
Weak |
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8U
,
8T
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Expression was less detectable in the mutant compared to wild type.
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Analysis Tools
