Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:147809
Masuda T, et al., Laser capture microdissection and cDNA array analysis for identification of mouse KIAA/FLJ genes differentially expressed in the embryonic dorsal spinal cord. Brain Res. 2009 Jan 16;1249:61-7
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3844026
|
|
Gene symbol:
|
Fstl5
|
|
Gene name:
|
follistatin-like 5
|
|
Probe:
|
Fstl5 probe1
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
Table 1:
embryonic day 10.5
(more  )
Specimen
Table 1:
(close  )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Strong |
Regionally restricted |
|
Expression was high in the dorsal spinal cord.
|
Specimen
3D left:
embryonic day 10.5
(more )
Specimen
3D left:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
3D ISH
|
Expression was detected in the dorsolateral edges of the spinal cord, especially in the dorsal root entry zone. Expression was also noted in the motor neurons.
|
| TS17: neural tube floor plate |
Present |
|
3D ISH
|
|
| TS17: dorsal root ganglion |
Present |
Regionally restricted |
3D ISH
|
Expression was detected in dorsal root ganglion neurons.
|
Specimen
3D right:
embryonic day 10.5
(more )
Note: Double labeled: blue - Fstl5; brown - Isl1 (assay MGI:3844024) and Is12 (assay MGI:3844025).
Specimen
3D right:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: blue - Fstl5; brown - Isl1 (assay MGI:3844024) and Is12 (assay MGI:3844025).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
3D ISH Islet-1/2
|
Expression was detected in the dorsolateral edges of the spinal cord, especially in the dorsal root entry zone. Expression was also noted in the motor neurons.
|
| TS17: neural tube floor plate |
Present |
|
3D ISH Islet-1/2
|
|
| TS17: dorsal root ganglion |
Present |
Regionally restricted |
3D ISH Islet-1/2
|
Expression was detected in dorsal root ganglion neurons.
|
Specimen
4D left:
embryonic day 12.5
(more )
Specimen
4D left:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
4D ISH
|
Expression was detected lateral to the ventricular zone.
|
| TS20: dorsal root ganglion |
Weak |
Regionally restricted |
4D ISH
|
Expression was weak in the dorsal root ganglion neurons.
|
Specimen
4D right:
embryonic day 12.5
(more )
Note: Double labeled: blue - Fstl5; brown - Isl1 (assay MGI:3844024) and Is12 (assay MGI:3844025).
Specimen
4D right:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: blue - Fstl5; brown - Isl1 (assay MGI:3844024) and Is12 (assay MGI:3844025).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
4D ISH Islet-1/2
|
Expression was detected lateral to the ventricular zone.
|
| TS20: dorsal root ganglion |
Weak |
Regionally restricted |
4D ISH Islet-1/2
|
Expression was weak in the dorsal root ganglion neurons.
|
|
Analysis Tools