Gene Expression Data
Assay Details
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Assay
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Reference:
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J:114487
Romanova LG, et al., Implication of Nucleolar Protein SURF6 in Ribosome Biogenesis and Preimplantation Mouse Development. Biol Reprod. 2006 Nov;75(5):690-6
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3692278
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Gene symbol:
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Surf6
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Gene name:
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surfeit gene 6
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Antibody:
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anti-SURF6
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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There is a variable degree of non-specific cytoplamic labeling in oocytes and early embryos.
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Results
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Specimen
1k:
postnatal
(more  )
Note: This is a germinal vesicle oocyte that lacks a perinucleolar chromatin rim. Double labeled: red - Surf6; green - Npm1 (assay MGI:3692191).
Specimen
1k:
(close  )
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Genetic Background:
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CF-1
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Age:
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postnatal
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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This is a germinal vesicle oocyte that lacks a perinucleolar chromatin rim. Double labeled: red - Surf6; green - Npm1 (assay MGI:3692191).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
Non-Uniform |
1k
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Expression is present in discrete foci at the surface of the nucleolus and within small patches attached to chromatin blocks.
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Specimen
1l:
embryonic day 1.8
(more )
Note: Late two-cell embryo. Double labeled: red - Surf6; green - Npm1 (assay MGI:3692191).
Specimen
1l:
(close )
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Genetic Background:
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involves: C57BL/6J * CF-1 * DBA/2J
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Age:
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embryonic day 1.8
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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Late two-cell embryo. Double labeled: red - Surf6; green - Npm1 (assay MGI:3692191).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
Non-Uniform |
1l
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Expression surrounds the nucleolar precursor body surface.
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Specimen
1m:
embryonic day 2.0
(more )
Note: Double labeled: red - Surf6; green - Npm1 (assay MGI:3692191).
Specimen
1m:
(close )
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Genetic Background:
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involves: C57BL/6J * CF-1 * DBA/2J
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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Double labeled: red - Surf6; green - Npm1 (assay MGI:3692191).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-cell stage embryo |
Present |
Non-Uniform |
1m
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Expression is clearly seen at the nucleolar precursor body periphery, where it forms a looser and more extended layer than was seen in two-cell embryos.
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Specimen
1n:
embryonic day 2.5
(more )
Note: Double labeled: red - Surf6; green - Npm1 (assay MGI:3692191).
Specimen
1n:
(close )
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Genetic Background:
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involves: C57BL/6J * CF-1 * DBA/2J
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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Double labeled: red - Surf6; green - Npm1 (assay MGI:3692191).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Present |
Non-Uniform |
1n
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Expression is clearly seen at the nucleolar precursor body periphery, where it forms a looser and more extended layer than was seen in two-cell embryos.
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Specimen
1o:
embryonic day 3.0
(more )
Note: Double labeled: red - Surf6; green - Npm1 (assay MGI:3692191).
Specimen
1o:
(close )
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Genetic Background:
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involves: C57BL/6J * CF-1 * DBA/2J
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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Double labeled: red - Surf6; green - Npm1 (assay MGI:3692191).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
Non-Uniform |
1o
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Expression is present in the nucleoli, which appear mature.
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Specimen
2:
embryonic day 1.0
(more )
Note: Mid two-cell embryo. Double labeled: red - Surf6; green - Coil (assay MGI:3692114).
Specimen
2:
(close )
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Genetic Background:
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involves: C57BL/6J * CF-1 * DBA/2J
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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Mid two-cell embryo. Double labeled: red - Surf6; green - Coil (assay MGI:3692114).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
Non-Uniform |
2b
,
2c
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Expression appears to be present in the Cajal (coiled) bodies, nuclear organelles.
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Analysis Tools